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. 1992 May 1;89(9):3990–3994. doi: 10.1073/pnas.89.9.3990

Anticodon-independent aminoacylation of an RNA minihelix with valine.

M Frugier 1, C Florentz 1, R Giegé 1
PMCID: PMC525617  PMID: 1570324

Abstract

Minihelices mimicking the amino acid acceptor and anticodon branches of yeast tRNA(Val) have been synthesized by in vitro transcription of synthetic templates. It is shown that a minihelix corresponding to the amino acid acceptor branch and containing solely a valine-specific identity nucleotide can be aminoacylated by yeast valyl-tRNA synthetase. Its charging ability is lost after mutating this nucleotide. This ability is stimulated somewhat by the addition of a second hairpin helix that mimicks the anticodon arm, which suggests that information originating from the anticodon stem-loop can be transmitted to the active site of the enzyme by the core of the protein.

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Selected References

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