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. 1978 Sep;27(3):483–489. doi: 10.1128/jvi.27.3.483-489.1978

Bacteriophage SPO1 development: defects in a gene 31 mutant.

A N Sarachu, M C Añón, O Grau
PMCID: PMC525835  PMID: 100606

Abstract

SPO1 temperature-sensitive mutant ts14-1, located in cistron 31, has a DD (DNA synthesis-delayed) phenotype at 37 degrees C and produces progeny in a stretched program. At 44 degrees C it behaves as a DO (DNA synthesis-defective) mutant and shuts off the viral RNA synthesis about 10 min after infection. The thermal sensitivity of this mutant is due to the inactivity of gp-31 (the product of gene 31) at 44 degrees C. However, gp-31 is synthesized at that temperature and partly recovers its activity at 37 degrees C. Only 5 min at the permissive temperature is enough to trigger the continuation of the phage program and to produce progeny. The partial defect at 37 degrees C and the expansion of the middle program together with the pleiotropic defects at the nonpermissive temperature could be suitable for the study of the controls involved in bacteriophage development.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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