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. 1978 Sep;27(3):560–567. doi: 10.1128/jvi.27.3.560-567.1978

Rebinding of transcriptase components (L and NS proteins) to the nucleocapsid template of vesicular stomatitis virus.

M G Mellon, S U Emerson
PMCID: PMC525842  PMID: 212581

Abstract

The L and NS proteins of vesicular stomatitis virions (New Jersey serotype) were solubilized with Triton X-100 and high-salt buffer and recombined with purified nucleocapsids under conditions similar to those used to reconstitute transcriptase activity in vitro. The nucleocapsid-bound L and NS proteins were separated from unbound proteins on a glycerol gradient. The rebinding of L and NS proteins mimics the in vivo binding in that at saturation the ratio of L and NS molecules to N molecules is approximately the same as observed in the intact virion. L and NS proteins were separated and added back independently and in combination to the template. The purified NS protein bound to the template in the absence of L protein. However, the L protein binding appeared to depend on the presence of NS protein. The presence of Mg2+ and nucleotides, which is required for transcription, was not necessary for the rebinding of L and NS proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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