Abstract
A new agent, provisionally designated Herpesvirus salmonis, was isolated from post-spawning rainbow trout (Salmo gairdneri) and studied primarily in the RTG-2 rainbow trout cell line. Infection of RTG-2 cells resulted in the formation of syncytia and Cowdry type A intranuclear inclusions. Replication occurred regularly at 5 and 10°C, but was inconsistent at 15°C, largely inhibited at 0°C, and completely inhibited at 20°C or higher. The virus was acid, heat, ether, and chloroform labile, but stable to freezing and thawing. It did not hemagglutinate. Viral DNA had a buoyant density of 1.709 g/cm3 and a guanine-cytosine value of 50%. Hexagonal nucleocapsids had a diameter of 90 nm and were first seen in nuclei at 36 h. Enveloped forms measured about 150 nm and occurred both cytoplasmically and extracellularly. At 10°C, a one-step growth culture required about 96 h; cell-associated virus peaked at about 105 PFU/ml and exceeded released virus by a factor of about 10.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Goodheart C. R., Plummer G. The densities of herpesviral DNAs. Prog Med Virol. 1975;19:324–352. [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUSSELL W. C., CRAWFORD L. V. SOME CHARACTERISTICS OF THE DEOXYRIBONUCLEIC ACID FROM HERPES SIMPLEX VIRUS. Virology. 1963 Nov;21:353–361. doi: 10.1016/0042-6822(63)90196-x. [DOI] [PubMed] [Google Scholar]
- STEMPAK J. G., WARD R. T. AN IMPROVED STAINING METHOD FOR ELECTRON MICROSCOPY. J Cell Biol. 1964 Sep;22:697–701. doi: 10.1083/jcb.22.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolf K., Darlington R. W. Channel catfish virus: a new herpesvirus of ictalurid fish. J Virol. 1971 Oct;8(4):525–533. doi: 10.1128/jvi.8.4.525-533.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolf K., Quimby M. C. Fish viruses: buffers and methods for plaquing eight agents under normal atmosphere. Appl Microbiol. 1973 Apr;25(4):659–664. doi: 10.1128/am.25.4.659-664.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolf K. The fish viruses. Adv Virus Res. 1966;12:35–101. doi: 10.1016/s0065-3527(08)60846-5. [DOI] [PubMed] [Google Scholar]