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. 1978 Sep;27(3):667–676. doi: 10.1128/jvi.27.3.667-676.1978

Characterization of the env gene in avian oncoviruses by heteroduplex mapping.

S F Hu, M M Lai, P K Vogt
PMCID: PMC525855  PMID: 212588

Abstract

The genome of ring-necked pheasant virus, an avian oncovirus, is largely homologous to the genomes of chicken oncoviruses except for a specific nonhomology in env, the gene coding for the surface glycoprotein of the virion (J. Tal, D. J. Fujita, S. Kawai, H. E. Varmus, and J. M. Bishop, J. Virol. 21:497--505, 1977). We have used this nonhomology between ring-necked pheasant virus and chicken oncoviruses in electron microscopic studies of heteroduplex molecules. The env-specific region of nonhomology is 1.5 to 1.7 kilobases in length. Its 3' boundary is located 0.6 to 0.7 kilobases from the 3' end of the genome in transformation-defective viruses and 2.5 kilobases from the 3' end in nondefective avian sarcoma viruses. Comparison of several strains of avian oncoviruses shows that the 3' half of this env region is conserved, while the 5' half is more diverged. A small area at the very 3' end of env also shows divergence between different avian oncoviruses. We found no evidence for the presence of a previously unrecognized gene between env and src. An electrophoretic comparison of the glycoproteins from various avian oncoviruses shows that those of ring-necked pheasant virus and Chinese quail virus differ in molecular weight from the glycoproteins of the chicken oncoviruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altaner C., Temin H. M. Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. Virology. 1970 Jan;40(1):118–134. doi: 10.1016/0042-6822(70)90384-3. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D. Tumor viruses: 1974. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1187–1200. doi: 10.1101/sqb.1974.039.01.137. [DOI] [PubMed] [Google Scholar]
  3. Bender W., Davidson N. Mapping of poly(A) sequences in the electron microscope reveals unusual structure of type C oncornavirus RNA molecules. Cell. 1976 Apr;7(4):595–607. doi: 10.1016/0092-8674(76)90210-5. [DOI] [PubMed] [Google Scholar]
  4. Bolognesi D. P., Montelaro R. C., Frank H., Schäfer W. Assembly of type C oncornaviruses: a model. Science. 1978 Jan 13;199(4325):183–186. doi: 10.1126/science.202022. [DOI] [PubMed] [Google Scholar]
  5. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  6. Casey J., Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4(5):1539–1552. doi: 10.1093/nar/4.5.1539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chen Y. C., Vogt P. K. Endogenous leukosis viruses in the avian family Phasianidae. Virology. 1977 Feb;76(2):740–750. doi: 10.1016/0042-6822(77)90255-0. [DOI] [PubMed] [Google Scholar]
  8. Duesberg P. H., Martin G. S., Vogt P. K. Glycoprotein components of avian and murine RNA tumor viruses. Virology. 1970 Aug;41(4):631–646. doi: 10.1016/0042-6822(70)90428-9. [DOI] [PubMed] [Google Scholar]
  9. Duesberg P. H. Physical properties of Rous Sarcoma Virus RNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1511–1518. doi: 10.1073/pnas.60.4.1511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Duff R. G., Vogt P. K. Characteristics of two new avian tumor virus subgroups. Virology. 1969 Sep;39(1):18–30. doi: 10.1016/0042-6822(69)90344-4. [DOI] [PubMed] [Google Scholar]
  11. Fujita D. J., Chen Y. C., Friis R. R., Vogt P. K. RNA tumor viruses of pheasants: characterization of avian leukosis subgroups F and G. Virology. 1974 Aug;60(2):558–571. doi: 10.1016/0042-6822(74)90350-x. [DOI] [PubMed] [Google Scholar]
  12. Galehouse D. M., Duesberg P. H. Glycoproteins of avian tumor virus recombinants: evidence for intragenic crossing-over. J Virol. 1978 Jan;25(1):86–96. doi: 10.1128/jvi.25.1.86-96.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hanafusa T., Hanafusa H. Isolation of leukosis-type virus from pheasant embryo cells: possible presence of viral genes in cells. Virology. 1973 Jan;51(1):247–251. doi: 10.1016/0042-6822(73)90388-7. [DOI] [PubMed] [Google Scholar]
  14. Joho R. H., Billeter M. A., Weissmann C. Mapping of biological functions on RNA of avian tumor viruses: location of regions required for transformation and determination of host range. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4772–4776. doi: 10.1073/pnas.72.12.4772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Junghans R. P., Hu S., Knight C. A., Davidson N. Heteroduplex analysis of avian RNA tumor viruses. Proc Natl Acad Sci U S A. 1977 Feb;74(2):477–481. doi: 10.1073/pnas.74.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Katz F. N., Rothman J. E., Lingappa V. R., Blobel G., Lodish H. F. Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3278–3282. doi: 10.1073/pnas.74.8.3278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kawai S., Hanafusa H. Isolation of defective mutant of avian sarcoma virus. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3493–3497. doi: 10.1073/pnas.70.12.3493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Keshet E., Temin H. M. Nucleotide sequences derived from pheasant DNA in the genome of recombinant avian leukosis viruses with subgroup F specificity. J Virol. 1977 Nov;24(2):505–513. doi: 10.1128/jvi.24.2.505-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kung H. J., Bailey J. M., Davidson N., Nicolson M. O., McAllister R. M. Structure, subunit composition, and molecular weight of RD-114 RNA. J Virol. 1975 Aug;16(2):397–411. doi: 10.1128/jvi.16.2.397-411.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  21. Lai M. M., Hu S. S. In vitro synthesis and characterisation of full- and half-genome length complementary DNA from avian oncoviruses. Nature. 1978 Feb 2;271(5644):481–483. doi: 10.1038/271481a0. [DOI] [PubMed] [Google Scholar]
  22. Lai M. M., Hu S. S., Vogt P. K. Occurrence of partial deletion and substitution of the src gene in the RNA genome of avian sarcoma virus. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4781–4785. doi: 10.1073/pnas.74.11.4781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lai M. M. Phosphoproteins of Rous sarcoma viruses. Virology. 1976 Oct 15;74(2):287–301. doi: 10.1016/0042-6822(76)90336-6. [DOI] [PubMed] [Google Scholar]
  24. Rothman J. E., Lenard J. Membrane asymmetry. Science. 1977 Feb 25;195(4280):743–753. doi: 10.1126/science.402030. [DOI] [PubMed] [Google Scholar]
  25. Shoyab M., Markham P. D., Baluda M. A. Host induced alteration of avian sarcoma virus B-77 genome. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1031–1035. doi: 10.1073/pnas.72.3.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tal J., Fujita D. J., Kawai S., Varmus H. E., Bishop J. M. Purification of DNA complementary to the env gene of avian sarcoma virus and analysis of relationships among the env genes of avian leukosis-sarcoma viruses. J Virol. 1977 Feb;21(2):497–505. doi: 10.1128/jvi.21.2.497-505.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vogt P. K. Envelope classification of avian RNA tumor viruses. Bibl Haematol. 1970;(36):153–167. doi: 10.1159/000391704. [DOI] [PubMed] [Google Scholar]
  28. Vogt P. K., Hu S. S. The genetic structure of RNA tumor viruses. Annu Rev Genet. 1977;11:203–238. doi: 10.1146/annurev.ge.11.120177.001223. [DOI] [PubMed] [Google Scholar]
  29. Wang L. H., Duesberg P. H., Kawai S., Hanafusa H. Location of envelope-specific and sarcoma-specific oligonucleotides on RNA of Schmidt-Ruppin Rous sarcoma virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):447–451. doi: 10.1073/pnas.73.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]

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