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. 1978 Sep;27(3):738–744. doi: 10.1128/jvi.27.3.738-744.1978

Evolution of bacteriophage phi X174. IV. Restriction enzyme cleavage map of St-1.

J N Grindley, G N Godson
PMCID: PMC525861  PMID: 702640

Abstract

The St-1 genome is about 6,050 base pairs in size, approximately 10% larger than phiX174 (5,375 base pairs). The DNA fragments obtained by HincII, HaeIII, and EcoRI digestion were ordered and aligned into a colinear map, and the single BglI cleavage site was located.

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Selected References

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  1. Barrell B. G., Air G. M., Hutchison C. A., 3rd Overlapping genes in bacteriophage phiX174. Nature. 1976 Nov 4;264(5581):34–41. doi: 10.1038/264034a0. [DOI] [PubMed] [Google Scholar]
  2. Bowes J. M., Dowell C. E. Purification and some properties of bacteriophage ST-1. J Virol. 1974 Jan;13(1):53–61. doi: 10.1128/jvi.13.1.53-61.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bowes J. M. Replication of bacteriophage ST-1 in Escherichia coli strains temperature sensitive in DNA synthesis. J Virol. 1974 Jun;13(6):1400–1403. doi: 10.1128/jvi.13.6.1400-1403.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradley D. E. A comparative study of some properties of the phi-X174 type bacteriophages. Can J Microbiol. 1970 Oct;16(10):965–971. doi: 10.1139/m70-165. [DOI] [PubMed] [Google Scholar]
  5. Chen C. Y., Hutchison C. A., 3rd, Edgell M. H. Isolation and genetic localization of three phi-X174 promoter regions. Nat New Biol. 1973 Jun 20;243(129):233–236. doi: 10.1038/newbio243233a0. [DOI] [PubMed] [Google Scholar]
  6. Derstine P. L., Dumas L. B., Miller C. A. Bacteriophage G4 DNA synthesis in temperature-sensitive dna mutants of Escherichia coli. J Virol. 1976 Sep;19(3):915–924. doi: 10.1128/jvi.19.3.915-924.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dumas L. B., Miller C. A. Inhibition of bacteriophage phi chi 174 DNA replication in dnaB mutants of Escherichia coli C. J Virol. 1974 Dec;14(6):1369–1379. doi: 10.1128/jvi.14.6.1369-1379.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edgell M. H., Hutchison C. A., 3rd, Sclair M. Specific endonuclease R fragments of bacteriophage phiX174 deoxyribonucleic acid. J Virol. 1972 Apr;9(4):574–582. doi: 10.1128/jvi.9.4.574-582.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Godson G. N., Boyer H. Susceptibility of the phiX-like phages G4 and G14 to R-EcoRi endonuclease. Virology. 1974 Nov;62(1):270–275. doi: 10.1016/0042-6822(74)90321-3. [DOI] [PubMed] [Google Scholar]
  10. Godson G. N. Evolution of phi chi 174. II. A cleavage map of the G4 phage genome and comparison with the cleavage map of phi chi 174. Virology. 1975 Feb;63(2):320–325. doi: 10.1016/0042-6822(75)90306-2. [DOI] [PubMed] [Google Scholar]
  11. Godson G. N. G4 DNA replication. III. Synthesis of replicative form. J Mol Biol. 1977 Dec 5;117(2):353–367. doi: 10.1016/0022-2836(77)90132-2. [DOI] [PubMed] [Google Scholar]
  12. Kranias E. G., Dumas L. B. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C. J Virol. 1974 Jan;13(1):146–154. doi: 10.1128/jvi.13.1.146-154.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee A. S., Sinsheimer R. L. A cleavage map of bacteriophage phiX174 genome. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2882–2886. doi: 10.1073/pnas.71.7.2882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  15. McFadden G., Denhardt D. T. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J Virol. 1974 Nov;14(5):1070–1075. doi: 10.1128/jvi.14.5.1070-1075.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SINSHEIMER R. L., STARMAN B., NAGLER C., GUTHRIE S. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962 Mar;4:142–160. doi: 10.1016/s0022-2836(62)80047-3. [DOI] [PubMed] [Google Scholar]
  17. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  18. Schekman R., Weiner A., Kornberg A. Multienzyme systems of DNA replication. Science. 1974 Dec 13;186(4168):987–993. doi: 10.1126/science.186.4168.987. [DOI] [PubMed] [Google Scholar]
  19. Smith H. O., Wilcox K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970 Jul 28;51(2):379–391. doi: 10.1016/0022-2836(70)90149-x. [DOI] [PubMed] [Google Scholar]
  20. Sugden B., De Troy B., Roberts R. J., Sambrook J. Agarose slab-gel electrophoresis equipment. Anal Biochem. 1975 Sep;68(1):36–46. doi: 10.1016/0003-2697(75)90676-4. [DOI] [PubMed] [Google Scholar]
  21. Taketo A. Host factor requirements and some properties of phiXtB. An evolutionary aspect of phiX-type phages. Mol Gen Genet. 1976 Oct 18;148(2):139–142. doi: 10.1007/BF00268377. [DOI] [PubMed] [Google Scholar]
  22. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  23. Wickner S., Hurwitz J. Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1053–1057. doi: 10.1073/pnas.73.4.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zechel K., Bouché J. P., Kornberg A. Replication of phage G4. A novel and simple system for the initiation of deoxyribonucleic acid synthesis. J Biol Chem. 1975 Jun 25;250(12):4684–4689. [PubMed] [Google Scholar]

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