Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1978 Sep;27(3):754–767. doi: 10.1128/jvi.27.3.754-767.1978

Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus.

H H Lee, L K Miller
PMCID: PMC525863  PMID: 359831

Abstract

A nuclear polyhedrosis virus (MNPV) isolated from a lepidopteran (Noctuidae) insect, Autographa californica, was cloned by successive plaque purification using virions containing only one nucleocapsid per envelope as inoculum. The ability to clone the virus by this method was demonstrated by the isolation of nondefective, genotypic variants of the virus with similar but not identical restriction endonuclease fragment patterns. Five distinct variants were identified by genotypic analysis with HindIII, EcoRI, SalI, and Bam HI restriction endonucleases. The characteristic genotype of each variant was maintained upon passage in insect larvae. The isolation of these virus variants demonstrates (i) the heterogeneity of the uncloned virus preparation and (ii) the ability to clone MNPVs by plaque purification of media-derived nonoccluded virions. The A. californica MNPV is being considered for commercial use as a pesticide in the United States, and the cloning of the virus, in view of the heterogeneity detected, may be advisable. The cloning and genotype analyses are also significant with regard to understanding the genetic nature of multiply embedded NPVs (those NPVs containing more than one nucleocapsid per envelope in the occluded form of the virus) and indicate that further genetic analysis of these viruses is possible.

Full text

PDF
754

Images in this article

757Image
on p.757
759Image
on p.759
760Image
on p.760
761Image
on p.761
762Image
on p.762
764Image
on p.764

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bud H. M., Kelly D. C. The DNA contained by nuclear polyhedrosis viruses isolated from four Spodoptera spp. (Lepidoptera, Noctuidae); genome size and configuration assessed by electron microscopy. J Gen Virol. 1977 Oct;37(1):135–143. doi: 10.1099/0022-1317-37-1-135. [DOI] [PubMed] [Google Scholar]
  2. David W. A. The status of viruses pathogenic for insects and mites. Annu Rev Entomol. 1975;20:97–117. doi: 10.1146/annurev.en.20.010175.000525. [DOI] [PubMed] [Google Scholar]
  3. Harrap K. A., Payne C. C., Robertson J. S. The properties of three baculoviruses from closely related hosts. Virology. 1977 Jun 1;79(1):14–31. doi: 10.1016/0042-6822(77)90330-0. [DOI] [PubMed] [Google Scholar]
  4. Henderson J. F., Faulkner P., MacKinnon E. A. Some biophysical properties of virus present in tissue cultures infected with the nuclear polyhedrosis virus of Trichoplusia ni. J Gen Virol. 1974 Jan;22(1):143–146. doi: 10.1099/0022-1317-22-1-143. [DOI] [PubMed] [Google Scholar]
  5. Kawanishi C. Y., Summers M. D., Stoltz D. B., Arnott H. J. Entry of an insect virus in vivo by fusion of viral envelope and microvillus membrane. J Invertebr Pathol. 1972 Jul;20(1):104–108. doi: 10.1016/0022-2011(72)90088-2. [DOI] [PubMed] [Google Scholar]
  6. Knudson D. L., Tinsley T. W. Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus. J Virol. 1974 Oct;14(4):934–944. doi: 10.1128/jvi.14.4.934-944.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller L. K., Dawes K. P. Restriction endonuclease analysis for the identification of baculovirus pesticides. Appl Environ Microbiol. 1978 Feb;35(2):411–421. doi: 10.1128/aem.35.2.411-421.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Potter K. N., Faulkner P., MacKinnon E. A. Strain selection during serial passage of Trichoplusia in nuclear polyhedrosis virus. J Virol. 1976 Jun;18(3):1040–1050. doi: 10.1128/jvi.18.3.1040-1050.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rohrmann G. F., Beaudreau G. S. Characterization of DNA from polyhedral inclusion bodies of the nucleopolyhedrosis single-rod virus pathogenic for Orgyia pseudotsugata. Virology. 1977 Dec;83(2):474–478. doi: 10.1016/0042-6822(77)90198-2. [DOI] [PubMed] [Google Scholar]
  10. Summers M. D., Anderson D. L. Characterization of nuclear polyhedrosis virus DNAs. J Virol. 1973 Dec;12(6):1336–1346. doi: 10.1128/jvi.12.6.1336-1346.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Summers M. D. Electron microscopic observations on granulosis virus entry, uncoating and replication processes during infection of the midgut cells of Trichoplusia ni. J Ultrastruct Res. 1971 Jun;35(5):606–625. doi: 10.1016/s0022-5320(71)80014-x. [DOI] [PubMed] [Google Scholar]
  12. Summers M. D., Volkman L. E. Comparison of biophysical and morphological properties of occluded and extracellular nonoccluded baculovirus from in vivo and in vitro host systems. J Virol. 1976 Mar;17(3):962–972. doi: 10.1128/jvi.17.3.962-972.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Volkman L. E., Summers M. D. Autographa californica nuclear polyhedrosis virus: comparative infectivity of the occluded, alkali-liberated, and nonoccluded forms. J Invertebr Pathol. 1977 Jul;30(1):102–103. doi: 10.1016/0022-2011(77)90045-3. [DOI] [PubMed] [Google Scholar]
  14. Volkman L. E., Summers M. D., Hsieh C. H. Occluded and nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization comparative infectivity, and in vitro growth studies. J Virol. 1976 Sep;19(3):820–832. doi: 10.1128/jvi.19.3.820-832.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES