Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1978 Sep;27(3):823–825. doi: 10.1128/jvi.27.3.823-825.1978

Mammalian retrovirus-associated RNase H is virus coded.

M H Lai, I M Verma, S R Tronick, S A Aaronson
PMCID: PMC525872  PMID: 81314

Abstract

RNase H of a temperature-sensitive mutant of Rauscher murine leukemia virus is thermolabile, establishing this activity as a virus-coded function of the mammalian type C virus reverse transcriptase.

Full text

PDF
823

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Grandgenett D. P., Gerard G. F., Green M. A single subunit from avian myeloblastosis virus with both RNA-directed DNA polymerase and ribonuclease H activity. Proc Natl Acad Sci U S A. 1973 Jan;70(1):230–234. doi: 10.1073/pnas.70.1.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Krakower J. M., Barbacid M., Aaronson S. A. Radioimmunoassay for mammalian type C viral reverse transcriptase. J Virol. 1977 May;22(2):331–339. doi: 10.1128/jvi.22.2.331-339.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Moelling K. Characterization of reverse transcriptase and RNase H from friend-murine leukemia virus. Virology. 1974 Nov;62(1):46–59. doi: 10.1016/0042-6822(74)90302-x. [DOI] [PubMed] [Google Scholar]
  4. Mölling K., Bolognesi D. P., Bauer H., Büsen W., Plassmann H. W., Hausen P. Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. Nat New Biol. 1971 Dec 22;234(51):240–243. doi: 10.1038/newbio234240a0. [DOI] [PubMed] [Google Scholar]
  5. Stephenson J. R., Aaronson S. A. Temperature-sensitive mutants of murine leukemia virus. 3. Mutants defective in helper functions for sarcoma virus fixation. Virology. 1974 Mar;58(1):294–297. doi: 10.1016/0042-6822(74)90163-9. [DOI] [PubMed] [Google Scholar]
  6. Stephenson J. R., Reynolds R. K., Aaronson S. A. Isolation of temperature-sensitive mutants of murine leukemia virus. Virology. 1972 Jun;48(3):749–756. doi: 10.1016/0042-6822(72)90158-4. [DOI] [PubMed] [Google Scholar]
  7. Tronick S. R., Stephenson J. R., Verma I. M., Aaronson S. A. Thermolabile reverse transcriptase of a mammalian leukemia virus mutant temperature sensitive in its replication and sarcoma virus helper functions. J Virol. 1975 Dec;16(6):1476–1482. doi: 10.1128/jvi.16.6.1476-1482.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Verma I. M., Baltimore D. Purification of the RNA-directed DNA polymerase from avian myeloblastosis virus and its assay with polynucleotide templates. Methods Enzymol. 1974;29:125–130. doi: 10.1016/0076-6879(74)29015-3. [DOI] [PubMed] [Google Scholar]
  9. Verma I. M., Mason W. S., Drost S. D., Baltimore D. DNA polymerase activity from two temperature-sensitive mutants of Rous sarcoma virus is thermolabile. Nature. 1974 Sep 6;251(5470):27–31. doi: 10.1038/251027a0. [DOI] [PubMed] [Google Scholar]
  10. Verma I. M. Studies on reverse transcriptase of RNA tumor viruses III. Properties of purified Moloney murine leukemia virus DNA polymerase and associated RNase H. J Virol. 1975 Apr;15(4):843–854. doi: 10.1128/jvi.15.4.843-854.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Verma I. M. Studies on reverse transcriptase of RNA tumor viruses. I. Localization of thermolabile DNA polymerase and RNase H activities on one polypeptide. J Virol. 1975 Jan;15(1):121–126. doi: 10.1128/jvi.15.1.121-126.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Verma I. M. The reverse transcriptase. Biochim Biophys Acta. 1977 Mar 21;473(1):1–38. doi: 10.1016/0304-419x(77)90005-1. [DOI] [PubMed] [Google Scholar]
  13. Verma I. M., Varmus H. E., Hunter E. Characterization of "early" temperature-sensitive mutants of avian sarcoma viruses: biological properties, thermolability of reverse transcriptase in vitro, and synthesis of viral DNA in infected cells. Virology. 1976 Oct 1;74(1):16–29. doi: 10.1016/0042-6822(76)90124-0. [DOI] [PubMed] [Google Scholar]
  14. Wang L. H., Duesberg P. H. DNA polymerase of murine sarcoma-leukemia virus: lack of detectable RNase H and low activity with viral RNA and natural DNA templates. J Virol. 1973 Dec;12(6):1512–1521. doi: 10.1128/jvi.12.6.1512-1521.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wu A. M., Sarngadharan M. G., Gallo R. C. Separation of ribonuclease H and RNA directed DNA polymerase (reverse transcriptase) of murine type-C RNA tumor viruses. Proc Natl Acad Sci U S A. 1974 May;71(5):1871–1876. doi: 10.1073/pnas.71.5.1871. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES