Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1978 Sep;27(3):826–830. doi: 10.1128/jvi.27.3.826-830.1978

Analysis of translational products of Friend strain of spleen focus-forming virus.

M Barbacid, D H Troxler, E M Scolnick, S A Aaronson
PMCID: PMC525873  PMID: 81315

Abstract

We have analyzed normal rat kidney cells nonproductively infected with the Friend strain of spleen focus-forming virus (SFFV) for the presence of murine leukemia virus-specific type C viral proteins. SFFV was found to code for the p15 and p12 proteins of Friend-murine leukemia virus as determined by immunological typing of their antigenic determinants. Molecular-size analysis of p15 and p12 proteins in SFFV nonproductively infected normal rat kidney cells indicated that these proteins are translated as parts of polyprotein molecules. The apparent molecular weights of the polypeptides bearing p12 antigenic determinants revealed the presence of translational products of the SFFV genome that could not be accounted for by know type C virus helper structural proteins.

Full text

PDF
826

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Bassin R. H., Weaver C. Comparison of murine sarcoma viruses in nonproducer and S + L - -transformed cells. J Virol. 1972 Apr;9(4):701–704. doi: 10.1128/jvi.9.4.701-704.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aaronson S. A., Stephenson J. R., Hino S., Tronick S. R. Differential expression of helper viral structural polypeptides in cells transformed by clonal isolates of woolly monkey sarcoma virus. J Virol. 1975 Nov;16(5):1117–1123. doi: 10.1128/jvi.16.5.1117-1123.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbacid M., Stephenson J. R., Aaronson S. A. Structural polypeptides of mammalian type C RNA viruses. Isolation and immunologic characterization of a low molecular weight polypeptide, p10. J Biol Chem. 1976 Aug 25;251(16):4859–4866. [PubMed] [Google Scholar]
  4. Barbacid M., Stephenson J. R., Aaronson S. A. gag Gene of mammalian type-C RNA tumour viruses. Nature. 1976 Aug 12;262(5569):554–559. doi: 10.1038/262554a0. [DOI] [PubMed] [Google Scholar]
  5. Bernstein A., Mak T. W., Stephenson J. R. The Friend virus genome: evidence for the stable association of MuLV sequences and sequences involved in erythroleukemic transformation. Cell. 1977 Sep;12(1):287–294. doi: 10.1016/0092-8674(77)90206-9. [DOI] [PubMed] [Google Scholar]
  6. Bister K., Hayman M. J., Vogt P. K. Defectiveness of avian myelocytomatosis virus MC29: isolation of long-term nonproducer cultures and analysis of virus-specific polypeptide synthesis. Virology. 1977 Oct 15;82(2):431–448. doi: 10.1016/0042-6822(77)90017-4. [DOI] [PubMed] [Google Scholar]
  7. Eckner R. J., Steeves R. A. A classification of the murine leukemia viruses. Neutralization of pseudotypes of Friend spleen focus-forming virus by type-specific murine antisera. J Exp Med. 1972 Oct 1;136(4):832–850. doi: 10.1084/jem.136.4.832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FRIEND C. Cell-free transmission in adult Swiss mice of a disease having the character of a leukemia. J Exp Med. 1957 Apr 1;105(4):307–318. doi: 10.1084/jem.105.4.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hino S., Stephenson J. R., Aaronson S. A. Radiommunoassays for the 70,000-molecular-weight glycoproteins of endogenous mouse type C viruses: viral antigen expression in normal mouse tissues and sera. J Virol. 1976 Jun;18(3):933–941. doi: 10.1128/jvi.18.3.933-941.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Khan A. S., Stephenson J. R. Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins. J Virol. 1977 Sep;23(3):599–607. doi: 10.1128/jvi.23.3.599-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Krakower J. M., Barbacid M., Aaronson S. A. Radioimmunoassay for mammalian type C viral reverse transcriptase. J Virol. 1977 May;22(2):331–339. doi: 10.1128/jvi.22.2.331-339.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lilly F., Steeves R. A. B-tropic Friend virus: a host-range pseudotype of spleen focus-forming virus (SFFV). Virology. 1973 Oct;55(2):363–370. doi: 10.1016/0042-6822(73)90176-1. [DOI] [PubMed] [Google Scholar]
  15. OLD L. J., BOYSE E. A., STOCKERT E. TYPING OF MOUSE LEUKAEMIAS BY SEROLOGICAL METHODS. Nature. 1964 Feb 22;201:777–779. doi: 10.1038/201777a0. [DOI] [PubMed] [Google Scholar]
  16. Parks W. P., Howk R. S., Anisowicz A., Scolnick E. M. Deletion mapping of moloney type C virus: polypeptide and nucleic acid expression in different transforming virus isolates. J Virol. 1976 May;18(2):491–503. doi: 10.1128/jvi.18.2.491-503.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parks W. P., Scolnick E. M. Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1766–1770. doi: 10.1073/pnas.69.7.1766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scolnick E. M., Goldberg R. J., Williams D. Characterizatiion of rat genetic sequences of Kirsten sarcoma virus: distinct class of endogenous rat type C viral sequences. J Virol. 1976 May;18(2):559–566. doi: 10.1128/jvi.18.2.559-566.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steeves R. A. Editorial: Spleen focus-forming virus in Friend and Rauscher leukemia virus preparations. J Natl Cancer Inst. 1975 Feb;54(2):289–297. doi: 10.1093/jnci/54.2.289. [DOI] [PubMed] [Google Scholar]
  20. Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Strand M., Wilsnack R., August J. T. Structural proteins of mammalian oncogenic RNA viruses: immunological characterization of the p15 polypeptide of Rauscher murine virus. J Virol. 1974 Dec;14(6):1575–1583. doi: 10.1128/jvi.14.6.1575-1583.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tronick S. R., Stephenson J. R., Aaronson S. A. Immunological characterization of a low molecular weight polypeptide of murine leukemia virus. Virology. 1973 Jul;54(1):199–206. doi: 10.1016/0042-6822(73)90129-3. [DOI] [PubMed] [Google Scholar]
  23. Troxler D. H., Boyars J. K., Parks W. P., Scolnick E. M. Friend strain of spleen focus-forming virus: a recombinant between mouse type C ecotropic viral sequences and sequences related to xenotropic virus. J Virol. 1977 May;22(2):361–372. doi: 10.1128/jvi.22.2.361-372.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Troxler D. H., Lowy D., Howk R., Young H., Scolnick E. M. Friend strain of spleen focus-forming virus is a recombinant between ecotropic murine type C virus and the env gene region of xenotropic type C virus. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4671–4675. doi: 10.1073/pnas.74.10.4671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Troxler D. H., Parks W. P., Vass W. C., Scolnick E. M. Isolation of a fibroblast nonproducer cell line containing the Friend strain of the spleen focus-forming virus. Virology. 1977 Feb;76(2):602–615. doi: 10.1016/0042-6822(77)90242-2. [DOI] [PubMed] [Google Scholar]
  26. Witte O. N., Rosenberg N., Paskind M., Shields A., Baltimore D. Identification of an Abelson murine leukemia virus-encoded protein present in transformed fibroblast and lymphoid cells. Proc Natl Acad Sci U S A. 1978 May;75(5):2488–2492. doi: 10.1073/pnas.75.5.2488. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES