Abstract
An unusual cytomegalovirus (CMV, strain Colburn) isolated from brain biopsy of a boy with clinical encephalopathy was studied for genetic relatedness to human and simian CMV. Cross-examination of the purified viral DNA by DNA-DNA reassociation kinetics analyses showed more than 90% homology between Colburn virus and simian CMV (strain GR2757) and a lack of detectable homology between Colburn virus and human CMV (strains AD-169 and TW-87). Restriction endonuclease analysis of Colburn DNA showed some similarity of the DNA fragment pattern with that of simian CMV DNA, although the DNA fragment patterns were not identical, and showed no similarity to that of human CMV DNA. The molecular size and density of viral DNA were close to those of simian CMV DNA. The antigenic study, as performed by complement fixation and neutralization tests, showed strong cross-reactivity of Colburn virus to simian GR2757 virus. One-way cross-reaction of Colburn virus to several human CMV isolates (AD-169, Davis, and Town) was detected by complement fixation; this one-way cross-reaction was not obvious in a plaque neutralization test. It was concluded that Colburn is a simian CMV-related virus.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dreesman G. R., Benyesh-Melnick M. Spectrum of human cytomegalovirus complement-fixing antigens. J Immunol. 1967 Dec;99(6):1106–1114. [PubMed] [Google Scholar]
- Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
- Hedgpeth J., Goodman H. M., Boyer H. W. DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448–3452. doi: 10.1073/pnas.69.11.3448. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huang E. S., Chen S. T., Pagano J. S. Human cytomegalovirus. I. Purification and characterization of viral DNA. J Virol. 1973 Dec;12(6):1473–1481. doi: 10.1128/jvi.12.6.1473-1481.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huang E. S., Kilpatrick B. A., Huang Y. T., Pagano J. S. Detection of human cytomegalovirus and analysis of strain variation. Yale J Biol Med. 1976 Mar;49(1):29–43. [PMC free article] [PubMed] [Google Scholar]
- Huang E. S., Pagano J. S. Human cytomegalovirus. II. Lack of relatedness to DNA of herpes simples I and II, Epstein-Barr virus, and nonhuman strains of cytomegalovirus. J Virol. 1974 Mar;13(3):642–645. doi: 10.1128/jvi.13.3.642-645.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huang Y. T., Huang E. S., Pagano J. S. Antisera to human cytomegaloviruses prepared in the guinea pig: specific immunofluorescence and complement fixation tests. J Immunol. 1974 Feb;112(2):528–532. [PubMed] [Google Scholar]
- Jovin T. M., Englund P. T., Bertsch L. L. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):2996–3008. [PubMed] [Google Scholar]
- Kilpatrick B. A., Huang E. S., Pagano J. S. Analysis of cytomegalovirus genomes with restriction endonucleases Hin D III and EcoR-1. J Virol. 1976 Jun;18(3):1095–1105. doi: 10.1128/jvi.18.3.1095-1105.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nigida S. M., Jr, Falk L. A., Wolfe L. G., Deinhardt F., Lakeman A., Alford C. A. Experimental infection of marmosets with a cytomegalovirus of human origin. J Infect Dis. 1975 Nov;132(5):582–586. doi: 10.1093/infdis/132.5.582. [DOI] [PubMed] [Google Scholar]
- Roy P. H., Smith H. O. DNA methylases of Hemophilus influenzae Rd. II. Partial recognition site base sequences. J Mol Biol. 1973 Dec 25;81(4):445–459. doi: 10.1016/0022-2836(73)90516-0. [DOI] [PubMed] [Google Scholar]
- Shah K. V., Ozer H. L., Ghazey H. N., Kelly T. J., Jr Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup. J Virol. 1977 Jan;21(1):179–186. doi: 10.1128/jvi.21.1.179-186.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogt V. M. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur J Biochem. 1973 Feb 15;33(1):192–200. doi: 10.1111/j.1432-1033.1973.tb02669.x. [DOI] [PubMed] [Google Scholar]
- Weiner L. P., Herndon R. M., Narayan O., Johnson R. T. Further studies of a simian virus 40-like virus isolated from human brain. J Virol. 1972 Jul;10(1):147–149. doi: 10.1128/jvi.10.1.147-149.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]