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. 1979 Dec;32(3):943–950. doi: 10.1128/jvi.32.3.943-950.1979

Transformation of primary rat kidney cells by DNA fragments of weakly oncogenic adenoviruses.

R Dijkema, B M Dekker, M J van der Feltz, A J van der Eb
PMCID: PMC525943  PMID: 513207

Abstract

Primary cultures of baby rat kidney (BRK) cells were transformed by intact DNA and DNA fragments of weakly oncogenic human adenovirus types 3 and 7. The smallest fragment found to contain transforming activity was the left-terminal 4% endo R.HindIII fragment (for both adenovirus type 3 and 7 DNAs). The efficiency of transformation of this fragment was low, and no permanent cell line could be established. Left-terminal fragments ranging from 84 to 4,5% of the viral genome could all transform BRK cells with the same efficiency as intact viral DNA. A number of adenovirus type 7 DNA fragment-transformed lines were established and were found to contain persistent viral DNA sequences and adenovirus subgroup B-specific T antigen. Consequently, the transforming functions of adenovirus types 3 and 7 are located at the extreme left-hand end of the genome, and the minimum size for a DNA fragment with transforming activity is 1.0 X 10(6) daltons. These results do not rule out the possibility that viral genes located outside the transforming region may also influence transformation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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