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. 1978 Mar;25(3):730–737. doi: 10.1128/jvi.25.3.730-737.1978

Roles of bacteriophage lambda gene products O and P during early and late phases of infection cycle.

J Klinkert, A Klein
PMCID: PMC525966  PMID: 642071

Abstract

Ring-to-ring (early) replication of bacteriophage lambda DNA was blocked after heat inactivation of the P protein. Rolling circle (late) replication continued for several rounds at the rate reached when the temperature shift was carried out. The same differential effect was observed after inhibition of RNA or protein synthesis during the two different phases of replication. In contrast, inactivation of the O protein resulted in a fast stop of lambda DNA synthesis at early and late times after infection. The results were consistent with the following interpretations. (i) The lambda P gene product plays a role in the initiation of the ring-to-ring replication. (ii) Ring-to-ring replication continues parallel to rolling circle replication, possibly diminishing with time after infection. (iii) The O function is stable in and necessary for the structural integrity of an elongation complex. It is unstable in free form and probably released from such a replication complex after each round of replication at the ring-to-ring stage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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