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. 1978 Mar;25(3):797–805. doi: 10.1128/jvi.25.3.797-805.1978

Primate retroviruses: immunological cross-reactivity between major structural proteins of new and old world primate virus isolates.

S G Devare, L O Arthur, D L Fine, J R Stephenson
PMCID: PMC525973  PMID: 76686

Abstract

The major 35,000-molecular-weight internal antigen (p35) of the squirrel monkey retrovirus (SMRV) was isolated and partially characterized. Immunological analysis of SMRV p35 led to the demonstration of antigenic determinants common to SMRV and the Mason-Pfizer monkey virus (MPMV). A broadly reactive competition immunoassay was developed utilizing antiserum to MPMV to precipitate 125I-labeled SMRV p35. Although the major structural proteins of MPMV and SMRV competed with equal efficiency in this assay, type B and type C oncornavirus proteins lacked detectable reactivity. Antibodies reactive with the major structural proteins of both MPMV and SMRV were observed in sera of several normal rhesus monkeys with known prior exposure to MPMV-infected animals. These findings demonstrate the ability of sera from naturally immunized primates to recognize broadly reactive interspecies antigenic determinants shared by the major structural proteins of type D oncornaviruses, and they suggest possible horizontal transmission of MPMV among rhesus monkeys. Although sera from a number of squirrel monkeys contained antibody to SMRV p35, the possibility that this latter reactivity was due to endogenous virus activation rather than horizontal transmission cannot be ruled out.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Stephenson J. R. Endogenous type-C RNA viruses of mammalian cells. Biochim Biophys Acta. 1976 Dec 23;458(4):323–354. doi: 10.1016/0304-419x(76)90006-8. [DOI] [PubMed] [Google Scholar]
  2. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
  3. Barbacid M., Stephenson J. R., Aaronson S. A. Evolutionary relationships between gag gene-coded proteins of murine and primate endogenous type C RNA viruses. Cell. 1977 Apr;10(4):641–648. doi: 10.1016/0092-8674(77)90097-6. [DOI] [PubMed] [Google Scholar]
  4. Barbacid M., Stephenson J. R., Aaronson S. A. Structural polypeptides of mammalian type C RNA viruses. Isolation and immunologic characterization of a low molecular weight polypeptide, p10. J Biol Chem. 1976 Aug 25;251(16):4859–4866. [PubMed] [Google Scholar]
  5. Benveniste R. E., Todaro G. J. Evolution of type C viral genes: evidence for an Asian origin of man. Nature. 1976 May 13;261(5556):101–108. doi: 10.1038/261101a0. [DOI] [PubMed] [Google Scholar]
  6. Charman H. P., Kim N., White M., Marquardt H., Gilden R. V., Kawakami T. Natural and experimentally induced antibodies to defined mammalian type-C virus proteins in primates. J Natl Cancer Inst. 1975 Dec;55(6):1419–1424. doi: 10.1093/jnci/55.6.1419. [DOI] [PubMed] [Google Scholar]
  7. Charman H. P., Rahman R., White M. H., Kim N., Gilden R. V. Radioimmunoassay for the major structural protein of Mason-Pfizer monkey virus: Attempts to detect the presence of antigen or antibody in humans. Int J Cancer. 1977 Apr 15;19(4):498–504. doi: 10.1002/ijc.2910190410. [DOI] [PubMed] [Google Scholar]
  8. Colcher D., Heberling R. L., Kalter S. S., Schlom J. Squirrel monkey retrovirus: an endogenous virus of a new world primate. J Virol. 1977 Aug;23(2):294–301. doi: 10.1128/jvi.23.2.294-301.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Devare S. G., Stephenson J. R. Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. J Virol. 1977 Aug;23(2):443–447. doi: 10.1128/jvi.23.2.443-447.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Devare S. G., Stephenson J. R., Sarma P. S., Aaronson S. A., Charder S. Bovine lymphosarcoma: development of a radioimmunologic technique for detection of the etiologic agent. Science. 1976 Dec 24;194(4272):1428–1430. doi: 10.1126/science.188129. [DOI] [PubMed] [Google Scholar]
  11. Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldberg R. J., Scolnick E. M., Parks W. P., Yakovleva L. A., Lapin B. A. Isolation of a primate type-C virus from a lymphomatous baboon. Int J Cancer. 1974 Dec 15;14(6):722–730. doi: 10.1002/ijc.2910140605. [DOI] [PubMed] [Google Scholar]
  13. Heberling R. L., Barker S. T., Kalter S. S., Smith G. C., Helmke R. J. Oncornavirus: isolation from a squirrel monkey (Saimiri sciureus) lung culture. Science. 1977 Jan 21;195(4275):289–292. doi: 10.1126/science.63993. [DOI] [PubMed] [Google Scholar]
  14. Kawakami T., Buckley P. M., McDowell T. S., DePaoli A. Antibodies to simian C-type virus antigen in sera of gibbons (Hylobates sp.). Nat New Biol. 1973 Nov 28;246(152):105–107. doi: 10.1038/newbio246105a0. [DOI] [PubMed] [Google Scholar]
  15. Khan A. S., Stephenson J. R. Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins. J Virol. 1977 Sep;23(3):599–607. doi: 10.1128/jvi.23.3.599-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kim B. S. Presence of antibody to a primate RNA virus in human plasma. Nature. 1975 Oct 16;257(5527):614–616. doi: 10.1038/257614a0. [DOI] [PubMed] [Google Scholar]
  17. Kurth R., Teich N. M., Weiss R., Oliver R. T. Natural human antibodies reactive with primate type-C viral antigens. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1237–1241. doi: 10.1073/pnas.74.3.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  19. Oroszlan S., Copeland T., Summers M. R., Smythers G., Gilden R. V. Amino acid sequence homology of mammalian type C RNA virus major internal proteins. J Biol Chem. 1975 Aug 25;250(16):6232–6239. [PubMed] [Google Scholar]
  20. Parks W. P., Scolnick E. M. Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1766–1770. doi: 10.1073/pnas.69.7.1766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Prochownik E. V., Kirsten W. H. Inhibition of reverse transcriptases of primate type C viruses by 7S immunoglobulin from patients with leukaemia. Nature. 1976 Mar 4;260(5546):64–67. doi: 10.1038/260064a0. [DOI] [PubMed] [Google Scholar]
  22. Schochetman G., Fine D., Arthur L., Gilden R., Heberling R. Characterization of a retravirus isolated from squirrel monkeys. J Virol. 1977 Aug;23(2):384–393. doi: 10.1128/jvi.23.2.384-393.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scolnick E. M., Parks W. P., Livingston D. M. Radioimmunoassay of mammalian type C viral proteins. I. Species specific reactions of murine and feline viruses. J Immunol. 1972 Sep;109(3):570–577. [PubMed] [Google Scholar]
  24. Stephenson J. R., Aaronson S. A. Endogenous C-type viral expression in primates. Nature. 1977 Mar 31;266(5601):469–472. doi: 10.1038/266469a0. [DOI] [PubMed] [Google Scholar]
  25. Stephenson J. R., Aaronson S. A. Search for antigens and antibodies crossreactive with type C viruses of the woolly monkeys and gibbon ape in animal models and in humans. Proc Natl Acad Sci U S A. 1976 May;73(5):1725–1729. doi: 10.1073/pnas.73.5.1725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stephenson J. R., Hino S., Garrett E. W., Aaronson S. A. Immunological cross reactivity of Mason-Pfizer monkey virus with type C RNA viruses endogenous to primates. Nature. 1976 Jun 17;261(5561):609–611. doi: 10.1038/261609a0. [DOI] [PubMed] [Google Scholar]
  27. Stephenson J. R., Reynolds R. K., Devare S. G., Reynolds F. H. Biochemical and immunological properties of gag genecoded structural proteins of endogenous tyep C RNA tumor viruses of diverse mammalian species. J Biol Chem. 1977 Nov 10;252(21):7818–7825. [PubMed] [Google Scholar]
  28. Todaro G. J., Benveniste R. E., Sherr C. J., Schlom J., Schidlovsky G., Stephenson J. R. Isolation and characterization of a new type D retrovirus from the asian primate, Presbytis obscurus (spectacled langur). Virology. 1978 Jan;84(1):189–194. doi: 10.1016/0042-6822(78)90231-3. [DOI] [PubMed] [Google Scholar]
  29. Todaro G. J., Sherr C. J., Benveniste R. E., Lieber M. M., Melnick J. L. Type C viruses of baboons: isolation from normal cell cultures. Cell. 1974 May;2(1):55–61. doi: 10.1016/0092-8674(74)90008-7. [DOI] [PubMed] [Google Scholar]
  30. Tronick S. R., Stephenson J. R., Aaronson S. A. Immunological characterization of a low molecular weight polypeptide of murine leukemia virus. Virology. 1973 Jul;54(1):199–206. doi: 10.1016/0042-6822(73)90129-3. [DOI] [PubMed] [Google Scholar]
  31. Yeh J., Ahmed M., Mayyasi S. A., Alessi A. A. Detection of an antigen related to Mason-Pfizer virus in malignant human breast tumors. Science. 1975 Nov 7;190(4214):583–584. doi: 10.1126/science.810888. [DOI] [PubMed] [Google Scholar]
  32. Yen J., Ahmed M., Lyles J., Larson D., Mayyasi S. A. Competition radioimmunoassay for mason-pfizer monkey virus: comparison with recent isolates. Int J Cancer. 1975 Apr 15;15(4):632–639. doi: 10.1002/ijc.2910150412. [DOI] [PubMed] [Google Scholar]

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