Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1978 Mar;25(3):806–810. doi: 10.1128/jvi.25.3.806-810.1978

Evidence from UV transcription mapping that late adenovirus type 2 mRNA is derived from a large precursor molecule.

S Goldberg, J Nevins, J E Darnell
PMCID: PMC525974  PMID: 642074

Abstract

The UV inactivation of labeling of various late adenovirus type 2 mRNA's is as great or greater than the inactivation of labeling of the same sequences that are present in a large primary RNA transcript in the cell nucleus. Thus, the large nuclear adenovirus type 2-specific RNA appears to be the obligatory precursor of cytoplasmic mRNA. The majority of the mRNA's derived from the large precursor apparently require the synthesis of the whole nuclear molecule. A UV-sensitive target necessary for proper processing of all the mRNA's may exist near the end of the transcription unit.

Full text

PDF
806

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachenheimer S., Darnell J. E. Hybridization of mRNA from adenovirus-transformed cells to segments of the adenovirus genome. J Virol. 1976 Jul;19(1):286–289. doi: 10.1128/jvi.19.1.286-289.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berget S. M., Moore C., Sharp P. A. Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171–3175. doi: 10.1073/pnas.74.8.3171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bräutigam A. R., Sauerbier W. Transcription unit mapping in bacteriophage T7. I. In vivo transcription by Escherichia coli RNA polymerase. J Virol. 1973 Oct;12(4):882–886. doi: 10.1128/jvi.12.4.882-886.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bräutigam A. R., Sauerbier W. Transcription unit mapping in bacteriophage T7. II. Proportionality of number of gene copies, mRNA, and gene product. J Virol. 1974 May;13(5):1110–1117. doi: 10.1128/jvi.13.5.1110-1117.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chinnadurai G., Rho H. M., Horton R. B., Green M. mRNA from the transforming segment of the adenovirus 2 genome in productively infected and transformed cells. J Virol. 1976 Oct;20(1):255–263. doi: 10.1128/jvi.20.1.255-263.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chow L. T., Gelinas R. E., Broker T. R., Roberts R. J. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA. Cell. 1977 Sep;12(1):1–8. doi: 10.1016/0092-8674(77)90180-5. [DOI] [PubMed] [Google Scholar]
  7. Dunn J. J., Studier F. W. T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3296–3300. doi: 10.1073/pnas.70.12.3296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans R. M., Fraser N., Ziff E., Weber J., Wilson M., Darnell J. E. The initiation sites for RNA transcription in Ad2 DNA. Cell. 1977 Nov;12(3):733–739. doi: 10.1016/0092-8674(77)90273-2. [DOI] [PubMed] [Google Scholar]
  9. Flint J. The topography and transcription of the adenovirus genome. Cell. 1977 Feb;10(2):153–166. doi: 10.1016/0092-8674(77)90211-2. [DOI] [PubMed] [Google Scholar]
  10. Gelinas R. E., Roberts R. J. One predominant 5'-undecanucleotide in adenovirus 2 late messenger RNAs. Cell. 1977 Jul;11(3):533–544. doi: 10.1016/0092-8674(77)90071-x. [DOI] [PubMed] [Google Scholar]
  11. Goldberg S., Schwartz H., Darnell J. E., Jr Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4520–4523. doi: 10.1073/pnas.74.10.4520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldberg S., Weber J., Darnell J. E., Jr The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping by effects of ultraviolet irradiation. Cell. 1977 Apr;10(4):617–621. doi: 10.1016/0092-8674(77)90094-0. [DOI] [PubMed] [Google Scholar]
  13. Hackett P. B., Sauerbier W. Radiological mapping of the ribosomal RNA transcription unit in E. coli. Nature. 1974 Oct 18;251(5476):639–641. doi: 10.1038/251639a0. [DOI] [PubMed] [Google Scholar]
  14. Hackett P. B., Sauerbier W. The transcriptional organization of the ribosomal RNA genes in mouse L cells. J Mol Biol. 1975 Jan 25;91(3):235–256. doi: 10.1016/0022-2836(75)90378-2. [DOI] [PubMed] [Google Scholar]
  15. Klessig D. F. Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions. Cell. 1977 Sep;12(1):9–21. doi: 10.1016/0092-8674(77)90181-7. [DOI] [PubMed] [Google Scholar]
  16. Lewis J. B., Atkins J. F., Anderson C. W., Baum P. R., Gesteland R. F. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344–1348. doi: 10.1073/pnas.72.4.1344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lindberg U., Persson T., Philipson L. Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection. J Virol. 1972 Nov;10(5):909–919. doi: 10.1128/jvi.10.5.909-919.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nevins J. R., Darnell J. E. Groups of adenovirus type 2 mRNA's derived from a large primary transcript: probable nuclear origin and possible common 3' ends. J Virol. 1978 Mar;25(3):811–823. doi: 10.1128/jvi.25.3.811-823.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sauerbier W., Millette R. L., Hackett P. B., Jr The effects of ultraviolet irradiation on the transcription of T4 DNA. Biochim Biophys Acta. 1970;209(2):368–386. doi: 10.1016/0005-2787(70)90735-5. [DOI] [PubMed] [Google Scholar]
  20. Wall R., Weber J., Gage Z., Darnell J. E. Production of viral mRNA in adenovirus- transformed cells by the post- transcriptional processing of heterogeneous nuclear RNA containing viral and cell sequences. J Virol. 1973 Jun;11(6):953–960. doi: 10.1128/jvi.11.6.953-960.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilson M. C., Sawicki S. G., Salditt-Georgieff M., Darnell J. E. Adenovirus type 2 mRNA in transformed cells: map positions and difference in transport time. J Virol. 1978 Jan;25(1):97–103. doi: 10.1128/jvi.25.1.97-103.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zimmer S. G., Raskas H. J. Synthesis of complementary viral transcripts early in productive infection with adenovirus 2. Virology. 1976 Mar;70(1):118–126. doi: 10.1016/0042-6822(76)90241-5. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES