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. 1978 Mar;25(3):923–927. doi: 10.1128/jvi.25.3.923-927.1978

Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity.

M McGrath, O Witte, T Pincus, I L Weissman
PMCID: PMC525988  PMID: 205680

Abstract

A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baird S., Raschke W., Weissman I. L. Evidence that MuLV-induced thymic lymphoma cells possess specific cell membrane binding sites for MuLV. Int J Cancer. 1977 Mar 15;19(3):403–413. doi: 10.1002/ijc.2910190319. [DOI] [PubMed] [Google Scholar]
  2. Duesberg P. H., Robinson H. L., Robinson W. S., Huebner R. J., Turner H. C. Proteins of Rous sarcoma virus. Virology. 1968 Sep;36(1):73–86. doi: 10.1016/0042-6822(68)90118-9. [DOI] [PubMed] [Google Scholar]
  3. Hung P. P., Robinson H. L., Robinson W. S. Isolation and characterization of proteins from Rous sarcoma virus. Virology. 1971 Jan;43(1):251–266. doi: 10.1016/0042-6822(71)90243-1. [DOI] [PubMed] [Google Scholar]
  4. Klement V., Rowe W. P., Hartley J. W., Pugh W. E. Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proc Natl Acad Sci U S A. 1969 Jul;63(3):753–758. doi: 10.1073/pnas.63.3.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Moennig V., Frank H., Hunsmann G., Schneider I., Schafer W. Properties of mouse leukemia viruses. VII. The major viral glycoprotein of friend leukemia virus. Isolation and physicochemical properties. Virology. 1974 Sep;61(1):100–111. doi: 10.1016/0042-6822(74)90245-1. [DOI] [PubMed] [Google Scholar]
  6. Rhim J. S., Williams L. B., Jr, Huebner R. J., Turner H. C. Concentration by Diaflo ultrafiltration of murine leukemia and sarcoma viruses grown in tissue cultures. Cancer Res. 1969 Jan;29(1):154–156. [PubMed] [Google Scholar]
  7. Robinson W. S., Pitkanen A., Rubin H. The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid. Proc Natl Acad Sci U S A. 1965 Jul;54(1):137–144. doi: 10.1073/pnas.54.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  9. Vogt P. K. DEAE-dextran: enhancement of cellular transformation induced by avian sarcoma viruses. Virology. 1967 Sep;33(1):175–177. doi: 10.1016/0042-6822(67)90109-2. [DOI] [PubMed] [Google Scholar]
  10. Witte O. N., Weissman I. L., Kaplan H. S. Structural characteristics of some murine RNA tumor viruses studied by lactoperoxidase iodination. Proc Natl Acad Sci U S A. 1973 Jan;70(1):36–40. doi: 10.1073/pnas.70.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Witte O. N., Weissman I. L. Membrane proteins of MSV-MLV: their role in virion-virion interactions in vitro. Virology. 1974 Oct;61(2):588–593. doi: 10.1016/0042-6822(74)90292-x. [DOI] [PubMed] [Google Scholar]
  12. Witte O. N., Weissman I. L. Polypeptides of Moloney sarcoma-leukemia virions: their resolution and incorporation into extracellular virions. Virology. 1974 Oct;61(2):575–587. doi: 10.1016/0042-6822(74)90291-8. [DOI] [PubMed] [Google Scholar]
  13. Yoshinaka Y., Hotta S. Purification of arboviruses grown in tissue culture. Proc Soc Exp Biol Med. 1971 Jul;137(3):1047–1053. doi: 10.3181/00379727-137-35725. [DOI] [PubMed] [Google Scholar]

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