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. 1978 Mar;25(3):928–932. doi: 10.1128/jvi.25.3.928-932.1978

RNA tumor virus phosphoproteins: primary structural analysis and identification of phosphopeptides.

B K Pal, M L Bryant, P Roy-Burman
PMCID: PMC525989  PMID: 205681

Abstract

Two-dimensional tryptic peptide mapping was used to compare the peptide sequences of the phosphoprotein (pp12) of cloned ecotropic and amphotropic wild mouse leukemia viruses, strains 1504 and 292. The maps of two ecotropic isolates were very similar to one another, as were the maps of two amphotropic isolates. There was also extensive similarity between the maps of this protein from ecotropic and amphotropic viruses, although characteristic peptide differences were readily recognized. These differences were consistent with the general type specificity of oncovirus phosphoproteins. The pp12 of the field isoalte of 292 virus contained five phosphopeptides, and the non-phosphorylated and variously phosphorylated species of this pp12 showed identical peptide maps, indicating differential phosphorylation of a single polypeptide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bryant M. L., Pal B. K., Gardner M. B., Elder J. H., Jensen F. C., Lerner R. A. Structural analysis of the major envelope glycoprotein (gp70) of the amphotropic and ecotropic type C viruses of wild mice. Virology. 1978 Feb;84(2):348–358. doi: 10.1016/0042-6822(78)90254-4. [DOI] [PubMed] [Google Scholar]
  2. Buchhagen D. L., Stutman O., Fleissner E. Chromatographic Separation and Antigenic Analysis of Proteins of the Oncornaviruses IV. Biochemical Typing of Murine Viral Proteins. J Virol. 1975 May;15(5):1148–1157. doi: 10.1128/jvi.15.5.1148-1157.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elder J. H., Jensen F. C., Bryant M. L., Lerner R. A. Polymorphism of the major envelope glycoprotein (gp70) of murine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family. Nature. 1977 May 5;267(5606):23–28. doi: 10.1038/267023a0. [DOI] [PubMed] [Google Scholar]
  4. Erikson E., Brugge J. S., Erikson R. L. Phosphorylated and nonphosphorylated forms of avian sarcoma virus polypeptide p19. Virology. 1977 Jul 1;80(1):177–185. doi: 10.1016/0042-6822(77)90390-7. [DOI] [PubMed] [Google Scholar]
  5. Glazer A. N. Specific chemical modification of proteins. Annu Rev Biochem. 1970;39:101–130. doi: 10.1146/annurev.bi.39.070170.000533. [DOI] [PubMed] [Google Scholar]
  6. Green R. W., Bolognesi D. P., Schäfer W., Pister L., Hunsmann G., De Noronha F. Polypeptides of mammalian oncornaviruses. I. Isolation and serological analysis polypeptides from murine and feline C-type viruses. Virology. 1973 Dec;56(2):565–579. doi: 10.1016/0042-6822(73)90058-5. [DOI] [PubMed] [Google Scholar]
  7. Hartley J. W., Rowe W. P. Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. J Virol. 1976 Jul;19(1):19–25. doi: 10.1128/jvi.19.1.19-25.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayman E. G., Pal B. K., Lai M. M., Roy-Burman P. Major phosphoprotein of avian sarcoma virus: peptide analysis of the variously charged species. Biochem Biophys Res Commun. 1977 Oct 24;78(4):1156–1161. doi: 10.1016/0006-291x(77)91414-0. [DOI] [PubMed] [Google Scholar]
  9. Hayman E. G., Pal B. K., Roy-Burman P. RNA tumour virus phosphoproteins: evidence for virus specificity of phosphorylation. J Gen Virol. 1977 Sep;36(3):459–469. doi: 10.1099/0022-1317-36-3-459. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lai M. M. Phosphoproteins of Rous sarcoma viruses. Virology. 1976 Oct 15;74(2):287–301. doi: 10.1016/0042-6822(76)90336-6. [DOI] [PubMed] [Google Scholar]
  12. Pal B. K., McAllister R. M., Gardner M. B., Roy-Burman P. Comparative studies on the structural phosphoproteins of mammalian type C viruses. J Virol. 1975 Jul;16(1):123–131. doi: 10.1128/jvi.16.1.123-131.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pal B. K., Roy-Burman P. Phosphoproteins: structural components of oncornaviruses. J Virol. 1975 Mar;15(3):540–549. doi: 10.1128/jvi.15.3.540-549.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pal B. K., Roy-Burman P. RNA tumor virus phosphoproteins: subvirion location of the multiple phosphorylated species. Virology. 1977 Dec;83(2):423–427. doi: 10.1016/0042-6822(77)90188-x. [DOI] [PubMed] [Google Scholar]
  15. Pal B. K., Wright M., Officer J. E., Gardner M. B., Roy-Burman P. Subviral components of a wild mouse embryo-derived type C oncornavirus. Virology. 1973 Nov;56(1):189–197. doi: 10.1016/0042-6822(73)90298-5. [DOI] [PubMed] [Google Scholar]
  16. Rasheed S., Gardner M. B., Chan E. Amphotropic host range of naturally occuring wild mouse leukemia viruses. J Virol. 1976 Jul;19(1):13–18. doi: 10.1128/jvi.19.1.13-18.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sen A., Sherr C. J., Todaro G. J. Phosphorylation of murine type C viral p12 proteins regulates their extent of binding to the homologous viral RNA. Cell. 1977 Mar;10(3):489–496. doi: 10.1016/0092-8674(77)90036-8. [DOI] [PubMed] [Google Scholar]
  18. Sen A., Sherr C. J., Todaro G. J. Specific binding of the type C viral core protein p12 with purified viral RNA. Cell. 1976 Jan;7(1):21–32. doi: 10.1016/0092-8674(76)90251-8. [DOI] [PubMed] [Google Scholar]
  19. Stephenson J. R., Reynolds R. K., Aaronson S. A. Comparisons of the immunological properties of two structural polypeptides of type C RNA viruses endogenous to old world monkeys. J Virol. 1976 Feb;17(2):374–384. doi: 10.1128/jvi.17.2.374-384.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tronick S. R., Stephenson J. R., Aaronson S. A. Immunological characterization of a low molecular weight polypeptide of murine leukemia virus. Virology. 1973 Jul;54(1):199–206. doi: 10.1016/0042-6822(73)90129-3. [DOI] [PubMed] [Google Scholar]

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