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. Author manuscript; available in PMC: 2018 Feb 20.
Published in final edited form as: Neuroscience. 2016 Dec 21;343:372–383. doi: 10.1016/j.neuroscience.2016.12.020

Fig 1. Localization of Wnt signaling.

Fig 1

The Tcf/LacZ transgenic Wnt reporter mice were used to localize cells that are responsive to Wnt3a stimulation. DAPI-stained nuclei are shown in white to demonstrate the retinal layers. Uninjured eyes had barely detectable basal Wnt signaling in the GCL, indicated by low levels of β-gal detection (green; arrows). However, increased expression of β-gal was detected in injured retinas that received saline injection (saline + injury), notably in the INL and GCL, and Wnt3a injection induced prominent Wnt signaling in the INL and GCL, indicated by increased immunostaining of β-gal (Wnt3a + injury). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar = 50μm