Table 4.
Electrophysiological properties of WT and mutant Cav3.2 channels.
| Cav3.2WT | Cav3.2196Leu | Cav3.21549Ile | Cav3.21951Glu | Cav3.22083Leu | |
|---|---|---|---|---|---|
| Activation V1/2 (mV) | − 43.81 ± 1.15 (14) | − 43.75 ± 1.06 (12) | − 53.47 ± 0.77 (9)⁎⁎⁎ | − 45.95 ± 1.33 (8) | − 42.42 ± 1.86 (9) |
| Activation slope (k, mV) | 5.89 ± 0.48 (14) | 6.62 ± 0.34 (12) | 6.21 ± 0.31 (9) | 5.69 ± 0.19 (8) | 5.99 ± 0.34 (9) |
| Inactivation V1/2 (mV) | − 67.55 ± 1.42 (13) | − 69.08 ± 1.47 (11) | − 76.58 ± 0.93 (6)⁎⁎ | − 67.98 ± 1.03 (6) | − 73.11 ± 2.65 (6) |
| Inactivation slope (k, mV) | 5.42 ± 0.36 (13) | 5.87 ± 0.42 (11) | 5.17 ± 0.22 (6) | 4.19 ± 0.13 (6) | 7.25 ± 0.64 (6)⁎⁎ |
| Activation kinetics @ − 30 mV (Tau act, ms) | 4.47 ± 0.33 (13) | 7.99 ± 0.41 (14)⁎⁎ | 8.30 ± 0.77 (9)⁎⁎ | 3.57 ± 0.22 (8) | 6.46 ± 0.86 (9) |
| Inactivation kinetics @ − 30 mV (Tau inact, ms) | 21.93 ± 2.09 (11) | 43.23 ± 3.14 (12)⁎⁎⁎ | 83.07 ± 8.47 (7)⁎⁎⁎ | 24.52 ± 2.86 (8) | 24.60 ± 2.23 (7) |
| Recovery (Tau, ms) | 509.76 ± 30.00 (8) | 419.12 ± 14.49 (9)⁎ | 1197.60 ± 93.92 (8)⁎⁎⁎ | 349.74 ± 15.63 (7)⁎⁎⁎ | 389.93 ± 28.89 (6)⁎⁎ |
| Deactivation kinetics @ − 90 mV (Tau deact, ms) | 3.01 ± 0.31 (15) | 3.08 ± 0.28 (13) | 11.67 ± 1.14 (9)⁎⁎⁎ | 2.50 ± 0.16 (12) | 3.07 ± 0.35 (9) |
| Facilitation (fold increase normalized current) | 1.12 ± 0.05 (8) | 1.34 ± 0.03 (8)⁎⁎⁎ | 1.07 ± 0.02 (6) | 1.26 ± 0.02 (9)⁎⁎ | 1.35 ± 0.05 (9)⁎⁎⁎ |
Number of recorded cells are indicated in brackets.
⁎
P < 0.05 compared with WT channels.
⁎⁎
P < 0.01 compared with WT channels.
⁎⁎⁎
P < 0.001 compared with WT channels.