Skip to main content
. Author manuscript; available in PMC: 2017 Jan 26.
Published in final edited form as: Laryngoscope. 2014 Jun 17;124(Suppl 5):S1–S12. doi: 10.1002/lary.24775

Figure 2.

Figure 2

To determine if delivery of high concentrations could account for the efficacy of non CAR non integrin delivery finding noted in Fig 1, adult macular explant cultures were set up and treated with 1 × 10 7 to 1 × 10 9 pu of native capsid vector and the Adf.DA vector which is incapable of binding CAR or integrins. Cultures were examined at 3 and 7 days post delivery using fluorescence microscopy. The Adf.DA vector showed only a very low level of transfection at 3 days post delivery that was independent of dose and overall transduced less cells/explant at the lowest dose of Adf delivered. By 7 days post delivery expression of GFP in the Adf.DA treated cultures was equivalent to the native capsid (Adf) treated cultures. This suggests that the kinetics of delivery is different.