Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Nov;172(11):6380–6385. doi: 10.1128/jb.172.11.6380-6385.1990

Bacillus subtilis mutants with alterations in ribosomal protein S4.

T M Henkin 1, G H Chambliss 1, F J Grundy 1
PMCID: PMC526823  PMID: 2121712

Abstract

Two mutants with different alterations in the electrophoretic mobility of ribosomal protein S4 were isolated as spore-plus revertants of a streptomycin-resistant, spore-minus strain of Bacillus subtilis. The mutations causing the S4 alterations, designated rpsD1 and rpsD2, were located between the argGH and aroG genes, at 263 degrees on the B. subtilis chromosome, distant from the major ribosomal protein gene cluster at 12 degrees. The mutant rpsD alleles were isolated by hybridization using a wild-type rpsD probe, and their DNA sequences were determined. The two mutants contained alterations at the same position within the S4-coding sequence, in a region containing a 12-bp tandem duplication; the rpsD1 allele corresponded to an additional copy of this repeated segment, resulting in the insertion of four amino acids, whereas the rpsD2 allele corresponded to deletion of one copy of this segment, resulting in the loss of four amino acids. The effects of these mutations, alone and in combination with streptomycin resistance mutations, on growth, sporulation, and streptomycin resistance were analyzed.

Full text

PDF
6380

Images in this article

6382Image
on p.6382
6383Image
on p.6383

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adoutte-Panvier A., Davies J. E., Gritz L. R., Littlewood B. S. Studies of ribosomal proteins of yeast species and their hybrids: gel electrophoresis and immunochemical cross-reactions. Mol Gen Genet. 1980;179(2):273–282. doi: 10.1007/BF00425454. [DOI] [PubMed] [Google Scholar]
  2. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boylan S. A., Suh J. W., Thomas S. M., Price C. W. Gene encoding the alpha core subunit of Bacillus subtilis RNA polymerase is cotranscribed with the genes for initiation factor 1 and ribosomal proteins B, S13, S11, and L17. J Bacteriol. 1989 May;171(5):2553–2562. doi: 10.1128/jb.171.5.2553-2562.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell K. M., Chambliss G. H. Streptomycin-resistant, asporogenous mutant of Bacillus subtilis. Mol Gen Genet. 1977 Dec 30;158(2):193–200. doi: 10.1007/BF00268313. [DOI] [PubMed] [Google Scholar]
  5. Carlton B. C. Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis. J Bacteriol. 1966 May;91(5):1795–1803. doi: 10.1128/jb.91.5.1795-1803.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Changchien L. M., Craven G. R. The function of the N-terminal region of ribosomal protein S4. J Mol Biol. 1976 Dec;108(2):381–401. doi: 10.1016/s0022-2836(76)80126-x. [DOI] [PubMed] [Google Scholar]
  7. Conrad R. C., Craven G. R. A cyanogen bromide fragment of S4 that specifically rebinds 16S RNA. Nucleic Acids Res. 1987 Dec 23;15(24):10331–10343. doi: 10.1093/nar/15.24.10331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ellwood M., Nomura M. Chromosomal locations of the genes for rRNA in Escherichia coli K-12. J Bacteriol. 1982 Feb;149(2):458–468. doi: 10.1128/jb.149.2.458-468.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Farabaugh P. J., Schmeissner U., Hofer M., Miller J. H. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli. J Mol Biol. 1978 Dec 25;126(4):847–857. doi: 10.1016/0022-2836(78)90023-2. [DOI] [PubMed] [Google Scholar]
  10. Gauthier A., Turmel M., Lemieux C. Mapping of chloroplast mutations conferring resistance to antibiotics in Chlamydomonas: evidence for a novel site of streptomycin resistance in the small subunit rRNA. Mol Gen Genet. 1988 Oct;214(2):192–197. doi: 10.1007/BF00337710. [DOI] [PubMed] [Google Scholar]
  11. Grundy F. J., Henkin T. M. Cloning and analysis of the Bacillus subtilis rpsD gene, encoding ribosomal protein S4. J Bacteriol. 1990 Nov;172(11):6372–6379. doi: 10.1128/jb.172.11.6372-6379.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henkin T. M., Campbell K. M., Chambliss G. H. Revertants of a streptomycin-resistant, oligosporogenous mutant of Bacillus subtilis. Mol Gen Genet. 1982;186(3):347–354. doi: 10.1007/BF00729453. [DOI] [PubMed] [Google Scholar]
  13. Henkin T. M., Chambliss G. H. Genetic analysis of a streptomycin-resistant oligosporogenous Bacillus subtilis mutant. J Bacteriol. 1984 Jan;157(1):202–210. doi: 10.1128/jb.157.1.202-210.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Henkin T. M., Chambliss G. H. Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4. Mol Gen Genet. 1984;193(2):364–369. doi: 10.1007/BF00330694. [DOI] [PubMed] [Google Scholar]
  15. Higo K., Otaka E., Osawa S. Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: correlation to Escherichia coli 30S proteins. Mol Gen Genet. 1982;185(2):239–244. doi: 10.1007/BF00330792. [DOI] [PubMed] [Google Scholar]
  16. Jarvis E. D., Widom R. L., LaFauci G., Setoguchi Y., Richter I. R., Rudner R. Chromosomal organization of rRNA operons in Bacillus subtilis. Genetics. 1988 Nov;120(3):625–635. doi: 10.1093/genetics/120.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LACKS S., HOTCHKISS R. D. A study of the genetic material determining an enzyme in Pneumococcus. Biochim Biophys Acta. 1960 Apr 22;39:508–518. doi: 10.1016/0006-3002(60)90205-5. [DOI] [PubMed] [Google Scholar]
  18. Lotti M., Dabbs E. R., Hasenbank R., Stöffler-Meilicke M., Stöffler G. Characterisation of a mutant from Escherichia coli lacking protein L15 and localisation of protein L15 by immuno-electron microscopy. Mol Gen Genet. 1983;192(3):295–300. doi: 10.1007/BF00392165. [DOI] [PubMed] [Google Scholar]
  19. Loughney K., Lund E., Dahlberg J. E. tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis. Nucleic Acids Res. 1982 Mar 11;10(5):1607–1624. doi: 10.1093/nar/10.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Melançon P., Lemieux C., Brakier-Gingras L. A mutation in the 530 loop of Escherichia coli 16S ribosomal RNA causes resistance to streptomycin. Nucleic Acids Res. 1988 Oct 25;16(20):9631–9639. doi: 10.1093/nar/16.20.9631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  22. Osawa S. Gene locus of a 30s ribosomal protein S20 of Bacillus subtilis. Mol Gen Genet. 1976 Feb 27;144(1):49–51. doi: 10.1007/BF00277303. [DOI] [PubMed] [Google Scholar]
  23. Powers T., Noller H. F. Dominant lethal mutations in a conserved loop in 16S rRNA. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1042–1046. doi: 10.1073/pnas.87.3.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rosset R., Gorini L. A ribosomal ambiguity mutation. J Mol Biol. 1969 Jan 14;39(1):95–112. doi: 10.1016/0022-2836(69)90336-2. [DOI] [PubMed] [Google Scholar]
  25. Schaeffer P., Millet J., Aubert J. P. Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A. 1965 Sep;54(3):704–711. doi: 10.1073/pnas.54.3.704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith I., Goldthwaite C., Dubnau D. The genetics of ribosomes in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1969;34:85–89. doi: 10.1101/sqb.1969.034.01.013. [DOI] [PubMed] [Google Scholar]
  27. Stern S., Powers T., Changchien L. M., Noller H. F. Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA. J Mol Biol. 1988 Jun 20;201(4):683–695. doi: 10.1016/0022-2836(88)90467-6. [DOI] [PubMed] [Google Scholar]
  28. Stern S., Weiser B., Noller H. F. Model for the three-dimensional folding of 16 S ribosomal RNA. J Mol Biol. 1988 Nov 20;204(2):447–481. doi: 10.1016/0022-2836(88)90588-8. [DOI] [PubMed] [Google Scholar]
  29. Stewart G. C., Wilson F. E., Bott K. F. Detailed physical mapping of the ribosomal RNA genes of Bacillus subtilis. Gene. 1982 Sep;19(2):153–162. doi: 10.1016/0378-1119(82)90001-4. [DOI] [PubMed] [Google Scholar]
  30. Suh J. W., Boylan S. A., Price C. W. Gene for the alpha subunit of Bacillus subtilis RNA polymerase maps in the ribosomal protein gene cluster. J Bacteriol. 1986 Oct;168(1):65–71. doi: 10.1128/jb.168.1.65-71.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wei Y. G., Surzycki S. J. Screening recombinant clones containing sequences homologous to Escherichia coli genes using single-stranded bacteriophage vector. Gene. 1986;48(2-3):251–256. doi: 10.1016/0378-1119(86)90083-1. [DOI] [PubMed] [Google Scholar]
  32. Yon J. R., Sammons R. L., Smith D. A. Cloning and sequencing of the gerD gene of Bacillus subtilis. J Gen Microbiol. 1989 Dec;135(12):3431–3445. doi: 10.1099/00221287-135-12-3431. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES