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. 1990 Oct;172(10):5686–5689. doi: 10.1128/jb.172.10.5686-5689.1990

Pyrophosphatase is essential for growth of Escherichia coli.

J Chen 1, A Brevet 1, M Fromant 1, F Lévêque 1, J M Schmitter 1, S Blanquet 1, P Plateau 1
PMCID: PMC526883  PMID: 2170325

Abstract

The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability.

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Selected References

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