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. 1991 Oct 15;88(20):9253–9256. doi: 10.1073/pnas.88.20.9253

Wegener autoantigen and myeloblastin are encoded by a single mRNA.

C Labbaye 1, P Musette 1, Y E Cayre 1
PMCID: PMC52692  PMID: 1681549

Abstract

Myeloblastin is a serine protease that has been identified in the human leukemia cell line HL-60. Down-regulation of this protease can inhibit proliferation and induce differentiation of promyelocyte-like human leukemic cells. Proteinase 3, a serine protease of human neutrophils, has been identified as the Wegener autoantigen. A high level of homology between myeloblastin and proteinase 3 has suggested that they may be a single serine protease. We have recently completed the 5'-terminal nucleotide sequence of proteinase 3 and shown that its mRNA was also expressed in HL-60 cells and in cells from patients with acute myeloid leukemia. Here we demonstrate that myeloblastin and proteinase 3 are encoded by a single mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bories D., Raynal M. C., Solomon D. H., Darzynkiewicz Z., Cayre Y. E. Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells. Cell. 1989 Dec 22;59(6):959–968. doi: 10.1016/0092-8674(89)90752-6. [DOI] [PubMed] [Google Scholar]
  2. Campanelli D., Melchior M., Fu Y., Nakata M., Shuman H., Nathan C., Gabay J. E. Cloning of cDNA for proteinase 3: a serine protease, antibiotic, and autoantigen from human neutrophils. J Exp Med. 1990 Dec 1;172(6):1709–1715. doi: 10.1084/jem.172.6.1709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carrell R. W. Alzheimer's disease. Enter a protease inhibitor. Nature. 1988 Feb 11;331(6156):478–479. doi: 10.1038/331478a0. [DOI] [PubMed] [Google Scholar]
  4. Cayre Y., Raynal M. C., Darzynkiewicz Z., Dorner M. H. Model for intermediate steps in monocytic differentiation: c-myc, c-fms, and ferritin as markers. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7619–7623. doi: 10.1073/pnas.84.21.7619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  6. Daniel F., Morello D., Le Bail O., Chambon P., Cayre Y., Kourilsky P. Structure and expression of the mouse beta 2-microglobulin gene isolated from somatic and non-expressing teratocarcinoma cells. EMBO J. 1983;2(7):1061–1065. doi: 10.1002/j.1460-2075.1983.tb01546.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gibson W. H., Burack S. L., Picciano A. The effects of serine protease inhibitors on morphological differentiation of murine neuroblastoma cells (NB15). J Cell Physiol. 1984 Apr;119(1):119–126. doi: 10.1002/jcp.1041190119. [DOI] [PubMed] [Google Scholar]
  8. Gupta S. K., Niles J. L., McCluskey R. T., Arnaout M. A. Identity of Wegener's autoantigen (p29) with proteinase 3 and myeloblastin. Blood. 1990 Nov 15;76(10):2162–2162. [PubMed] [Google Scholar]
  9. Heidmann O., Rougeon F. Multiple sequences related to a constant-region kappa light chain gene in the rabbit genome. Cell. 1982 Mar;28(3):507–513. doi: 10.1016/0092-8674(82)90205-7. [DOI] [PubMed] [Google Scholar]
  10. Jenne D. E., Tschopp J., Lüdemann J., Utecht B., Gross W. L. Wegener's autoantigen decoded. Nature. 1990 Aug 9;346(6284):520–520. doi: 10.1038/346520a0. [DOI] [PubMed] [Google Scholar]
  11. Kao R. C., Wehner N. G., Skubitz K. M., Gray B. H., Hoidal J. R. Proteinase 3. A distinct human polymorphonuclear leukocyte proteinase that produces emphysema in hamsters. J Clin Invest. 1988 Dec;82(6):1963–1973. doi: 10.1172/JCI113816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koeffler H. P. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood. 1983 Oct;62(4):709–721. [PubMed] [Google Scholar]
  13. Kozak M. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo. Nature. 1984 Mar 15;308(5956):241–246. doi: 10.1038/308241a0. [DOI] [PubMed] [Google Scholar]
  14. Loh E. Y., Elliott J. F., Cwirla S., Lanier L. L., Davis M. M. Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. Science. 1989 Jan 13;243(4888):217–220. doi: 10.1126/science.2463672. [DOI] [PubMed] [Google Scholar]
  15. Lüdemann J., Utecht B., Gross W. L. Anti-neutrophil cytoplasm antibodies in Wegener's granulomatosis recognize an elastinolytic enzyme. J Exp Med. 1990 Jan 1;171(1):357–362. doi: 10.1084/jem.171.1.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Musette P., Labbaye C., Dorner M. H., Cayre Y. E., Casanova J. L., Kourilsky P. Wegener's autoantigen and leukemia. Blood. 1991 Mar 15;77(6):1398–1399. [PubMed] [Google Scholar]
  17. Niles J. L., McCluskey R. T., Ahmad M. F., Arnaout M. A. Wegener's granulomatosis autoantigen is a novel neutrophil serine proteinase. Blood. 1989 Nov 1;74(6):1888–1893. [PubMed] [Google Scholar]
  18. Rao N. V., Wehner N. G., Marshall B. C., Gray W. R., Gray B. H., Hoidal J. R. Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Structural and functional properties. J Biol Chem. 1991 May 25;266(15):9540–9548. [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Sullivan L. M., Quigley J. P. An anticatalytic monoclonal antibody to avian plasminogen activator: its effect on behavior of RSV-transformed chick fibroblasts. Cell. 1986 Jun 20;45(6):905–915. doi: 10.1016/0092-8674(86)90565-9. [DOI] [PubMed] [Google Scholar]
  21. Wilde C. G., Snable J. L., Griffith J. E., Scott R. W. Characterization of two azurphil granule proteases with active-site homology to neutrophil elastase. J Biol Chem. 1990 Feb 5;265(4):2038–2041. [PubMed] [Google Scholar]
  22. von Heijne G. Signal sequences. The limits of variation. J Mol Biol. 1985 Jul 5;184(1):99–105. doi: 10.1016/0022-2836(85)90046-4. [DOI] [PubMed] [Google Scholar]

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