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. 1990 Oct;172(10):6160–6161. doi: 10.1128/jb.172.10.6160-6161.1990

Selection of Acinetobacter calcoaceticus mutants deficient in the p-hydroxybenzoate hydroxylase gene (pobA), a member of a supraoperonic cluster.

G B Hartnett 1, B Averhoff 1, L N Ornston 1
PMCID: PMC526948  PMID: 2211534

Abstract

p-Hydroxybenzoate hydroxylase, the product of the pobA gene, gives rise to protocatechuate, which is metabolized by enzymes encoded by the pca operon in Acinetobacter calcoaceticus. Mutations in pcaD prevented growth of A. calcoaceticus with succinate in the presence of p-hydroxybenzoate. Mutants selected on this medium contained the original mutation in pcaD and also carried spontaneous mutations in pobA. These independently expressed genes were cotransformed with a frequency of 15% and thus are components of a supraoperonic cluster.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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