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. 1991 Oct 15;88(20):9267–9271. doi: 10.1073/pnas.88.20.9267

Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor.

D Maglione 1, V Guerriero 1, G Viglietto 1, P Delli-Bovi 1, M G Persico 1
PMCID: PMC52695  PMID: 1924389

Abstract

A human cDNA coding for a protein related to the vascular permeability factor (VPF) was isolated from a term placenta cDNA library; we therefore named its product placenta growth factor (PlGF). PlGF is a 149-amino-acid-long protein and is highly homologous (53% identity) to the platelet-derived growth factor-like region of human VPF. Computer analyses reveal a putative signal peptide and two probable N-glycosylation sites in the PlGF protein, one of which is also conserved in human VPF. By using N-glycosidase F, tunicamycin, and specific antibodies produced in both chicken and rabbit, we demonstrate that PlGF, derived from transfected COS-1 cells, is actually N-glycosylated and secreted into the medium. In addition, PlGF, like VPF, proves to be a dimeric protein. Finally, a conditioned medium from COS-1 cells containing PlGF is capable of stimulating specifically the growth of CPA, a line of endothelial cells, in vitro.

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