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. 2016 Nov 18;129(4):405–414. doi: 10.1182/blood-2016-06-721571

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Figure 5. — Bmp6^fl/fl;Tek-Cre+ mice exhibit extrahepatic iron loading, whereas Bmp6^fl/fl;Alb-Cre+ and Bmp6^fl/fl;LysM-Cre+ mice do not. Eight- and 16-week-old littermate male and female Bmp6^+/+, Bmp6^+/−, and Bmp6^−/− global knockout mice and Bmp6 conditional knockout mice in ECs (Bmp6^fl/fl;Tek-Cre+), macrophages (Bmp6^fl/fl;LysM-Cre+), and hepatocytes (Bmp6^fl/fl;Alb-Cre+) compared with littermate controls (Bmp6^fl/fl;Cre–) from Figure 4 were analyzed for tissue iron in (A,C) pancreas and (B,C) heart by (A-B) biochemical analysis or (C) Perls’ Prussian blue. Diaminobenzidine (DAB)-enhanced Perls’ stain was performed on sections that appeared negative or faint by standard Perls’ Prussian blue. n = 4 to 7 mice per group (supplemental Table 2), with tissues from 1 representative mouse shown in (C) (original magnification ×20; scale bar represents 100 μM; boxed areas shown enlarged ×5 for pancreas and ×3 for heart in insets for some panels). **P < .01, ***P < .001 relative to control Bmp6^+/+ mice by one-way ANOVA with Dunnett’s post hoc test; +P < .05, ++P < .01 relative to control Bmp6^fl/fl;Cre– mice by Student t test.