Figure 1.

Characterisation of platelets from Cttn KO and DKO mice. (A) Western blots of platelet lysates confirming phosphorylation of Cttn downstream of G-protein coupled and tyrosine kinase linked receptors. Cortactin was immuno-precipitated from stimulated mouse platelet lysates and western blotted with anti-phosphotyrosine (4G10) and anti-cortactin (4F11) antibodies. (B) Whole blood platelet counts and mean platelet volumes were measured from WT, Cttn and DKO mice. No significant difference was observed between the genotypes in either platelet number or volume. Data is mean ± SD (WT, n = 37; Cttn KO, n = 24; DKO, n = 18). (C) Platelet surface receptor levels were analysed by flow cytometry. No significant differences were observed between either genotype and WT controls. Data are % of WT control (Mean ± SEM; n = 3) and values were corrected for IgG background staining.