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. 2017 Jan 30;7:41437. doi: 10.1038/srep41437

Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells

Yu-Huei Liu 1,2, Yui-Ping Weng 3,4, Hsuan-Yuan Lin 5, Sai-Wen Tang 6, Chao-Jung Chen 1, Chi-Jung Liang 6, Chung-Yu Ku 6, Jung-Yaw Lin 5,6,a
PMCID: PMC5278379  PMID: 28134285

Abstract

Hepatocellular carcinoma (HCC) remains the leading cause of cancer mortality with limited therapeutic targets. The endoplasmic reticulum (ER) plays a pivotal role in maintaining proteostasis in normal cells. However, alterations in proteostasis are often found in cancer cells, making it a potential target for therapy. Polygonum bistorta is used in traditional Chinese medicine owing to its anticancer activities, but the molecular and pharmacological mechanisms remain unclear. Using hepatoma cells as a model system, this study demonstrated that P. bistorta aqueous extract (PB) stimulated ER stress by increasing autophagosomes but by blocking degradation, followed by the accumulation of ubiquitinated proteins and cell apoptosis. In addition, an autophagy inhibitor did not enhance ubiquitinated protein accumulation whereas a reactive oxygen species (ROS) scavenger diminished both ubiquitinated protein accumulation and ligand-stimulated epidermal growth factor receptor (EGFR) expression, suggesting that ROS generation by PB may be upstream of PB-triggered cell death. Nevertheless, PB-exerted proteostasis impairment resulted in cytoskeletal changes, impairment of cell adhesion and motility, and inhibition of cell cycle progression. Oral administration of PB delayed tumour growth in a xenograft model without significant body weight loss. These findings indicate that PB may be a potential new alternative or complementary medicine for HCC.

Hepatocellular carcinoma (HCC) remains the leading cause of cancer mortality worldwide1,2,3,4,5. HCC patients usually present in advanced stages when surgical resection and/or chemical embolism are no longer feasible. Few chemotherapies and targeted therapies are capable of killing HCC. Therefore, new directions and new interventions are unmet urgent needs for HCC.

The endoplasmic reticulum (ER) plays an important role in maintaining proteostasis6. The accumulation of misfolded proteins in the ER initiates a protective unfolded protein response (UPR) in the cell in response to ER stress7. The major function of UPR is to modulate proteostasis through translational attenuation and upregulation of genes encoding ER chaperone proteins and secretory machinery to increase the protein-folding capacity of the ER8,9. However, persistent or intense stress will drive these unfolding proteins to translocate to the cytoplasm, where they are degraded through the ubiquitin-proteasome system (UPS). Once the UPS fails, death-associated protein kinase (DAPK), an upstream integrator of apoptosis and autophagy in response to ER stress, will be triggered10,11.

A significant drift has occurred in recent years towards the use of medicinal plants to manage/treat many debilitating diseases, including HCC12,13. PHY906, a 1800-year-old Chinese herbal formula, potentiates chemotherapy effects through improving multiple mechanisms, including the inflammatory state and tumour microenvironment4,14,15,16,17,18,19 and has been evaluated in clinical studies in colorectal cancer20,21, pancreatic cancer22,23, and is awaiting FDA approval for HCC24. The root and rhizome of Polygonum bistorta (pronounced quansen) has been a widely used Chinese medicine for more than 1000 years for treating exogenous heat, cough, dysentery, sepsis, haematemesis (blood heat bleeding), as well as inflammatory conditions such as carbuncle swelling and pain. Recent studies suggested that some organic solvent fractions of P. bistorta showed promise for the inhibition of xanthine oxidase activity25, therapeutic effects in snakebites26, ionophore-induced allergies27, retina ischemia/reperfusion injury28, and growth inhibition of various cancer cell lines including HCC lines in vitro29,30. Other new formulas including P. bistorta such as Zeng-Sheng-Ping (Antitumor B) for mutagen-induced lung cancer and oral cancer31,32, and Fei-Liu-Ping for lung cancer metastasis33,34, have claimed to be successful in the clinic for more than 20 years. However, the molecular and pharmacological mechanisms of P. bistorta require further investigation.

In the present study, we examined the efficacy of an aqueous extract of P. bistorta (PB) against hepatoma cells in vitro, as well as its antitumor effects on transplanted tumours in mice. We also demonstrate the anti-tumour mechanism of PB is mediated by reactive oxygen species (ROS), ER stress, autophagy, and proteasome degradation inhibition, which results in apoptosis.

Results

PB induces autophagy in Hep3B cells

Chemical profiling indicated that the quantities of gallic acid (GA), 3,4-dihydroxybenzoic acid (DHBA), and chlorogenic acid (CA), in PB were 2,091.4 ± 12.5 ppb, 162.6 ± 7.6 ppb, and 1,757.7 ± 170.7 ppb respectively, and were used as indicators for PB (Fig. 1). As shown in Fig. 2A, PB significantly decreased Hep3B and HepG2 cell survival in a dose-dependent manner. However, PB exhibited limited killing potency in mouse hepatocytes with identical treatment periods and concentrations. Administration of the polyphenols GA, DHBA, and CA at corresponding doses, either alone or in combination, did not show significant cytotoxic effects (data not shown), indicating that the complete PB extract is required to induce the cytotoxic effects.

Figure 1. Chemical identification of polyphenols in PB.

Figure 1

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Chemical identification of polyphenols gallic acid (GA), 3,4-dihydroxybenzoic acid (DHBA), and chlorogenic acid (CA) in PB.

Figure 2. PB induces autophagy but blocks autophagosome trafficking and autophagosome-lysosome fusion in Hep3B cells.

Figure 2

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(A) Hep3B cells, HepG2 cells, and primary mouse hepatocytes were treated with increasing concentrations of PB (6–2000 μg/mL) for 24 h; viable cells were quantified using the MTT assay. Data are presented as mean ± SD from at least 3 independent experiments. Bars = SD. *P < 0.05, **P < 0.01, ***P < 0.001, control versus PB-treated cells. (B) Hep3B cells were treated for 6, 24 and 48 h with the indicated concentrations of PB. Levels of protein expression were analysed by Western blot using antibodies against LC3B, p62/SQSTM1, ATG12, BECN1 and Actin. Images were cropped from different blots run under the same experimental conditions. The original blots were attached as Supplementary Figure 3A. (C) Hep3B cells transfected with GFP-LC3B were treated with 240 μg/mL PB for 24 h and then observed under a confocal microscope. (D) Hep3B cells were treated for 24 h with the indicated concentrations of PB. Levels of protein expression were analysed by Western blot using antibodies against phosphorylated and total mTOR, 4EBP1, and p70S6K1 as well as actin. Images were cropped from different blots run under the same experimental conditions. The original blots were attached as Supplementary Figure 3B. (E) Hep3B cells were serum-starved overnight, and incubated with or without PB (240 μg/mL) for 6 h before stimulating with 20 ng/mL EGF for 20 min. EGFR protein levels were analysed by confocal microscope. (F) Hep3B cells were treated PB for 12, 24 or 48 h with indicated concentrations. Levels of protein expression were analysed by Western blot using antibodies against EGFR and Actin. Images were cropped from different blots run under the same experimental conditions in each panel. The original blots were attached as Supplementary Figure 3C.

The conversion from microtubule-associated protein 1 light chain 3b (MAP1LC3B/LC3B) (LC3B-I) to phosphatidylethanolamine covalent conjugated LC3B-II is a hallmark of autophagy35. To determine whether PB induced autophagy, the abundance of LC3B-I was monitored. As shown in Fig. 2B, PB increased the expression level of LC3B-II protein in a dose- and time-dependent manner in Hep3B cells. In addition, by monitoring autophagosome formation using a plasmid encoding GFP-LC3B fusion protein in Hep3B cells, GFP-LC3B puncta were observed in PB treated cells, indicating that PB treatment enhanced autophagosome accumulation in cells (Fig. 2C).

PB facilitates autophagosome formation more rapidly than autolysosome formation in Hep3B cells

Autophagy is an intracellular process for the degradation of cytoplasmic contents by autolysosome fusion from isolated membrane (phagophore)-elongated autophagosomes and lysosomes36. Autophagosome accumulation in PB-treated cells reflects an imbalance between their rate of formation and degradation37, which could be explained by the promotion or impairment of autophagic flux. When examined using Western blot analyses, p62/SQSTM1, a marker to determine formation of autophagosome, was persistently increased in cells treated with PB at 6 h and 24 h, but not at the 48 h time point, implying that PB facilitated the accumulation of autophagosomes at least within 24 h of treatment. However, the expression level of an essential autophagic protein which mediates the maturation of autolysosome, beclin 1 (BECN1)36, was decreased by PB at all treatment concentrations and time points, indicating that autolysosome formation may be impaired by PB (Fig. 2B). Furthermore, when tracing the signalling for autophagosome initiation, we found that PB downregulated phosphorylated (p)-mechanistic target of rapamycin serine/threonine kinase (mTOR) and the surrogate measures of mTOR activity, p-p70S6K1 levels and expression of 4EBP1, indicating the involvement of mTOR inactivation by PB (Fig. 2D). These results demonstrated that PB downregulates mTOR activity to facilitate autophagosome formation more rapidly than autolysosome formation in Hep3B cells.

The impairment of autolysosome formation will cause cellular macromolecule proteolytic degradation dysfunction. Interference of autophagosome-lysosome fusion by sequestering two organelles in different localisations may be one cause for this impairment. Epidermal growth factor receptor (EGFR) is a typical receptor tyrosine kinase that is endocytosed and delivered from the cell surface to lysosomes for degradation after EGF binding-induced activation. To monitor lysosome localisation and activity, EGFR was chosen as an endogenous marker. Hep3B cells were first treated for 6 h with or without PB, and then EGF was added for 20 min. The localisation of EGFR was then observed. Interestingly, EGF-induced EGFR degradation was observed in the absence of PB treatment. However, EGFR appeared to translocate into the nucleus, implying that the dislocation of the lysosomes interfered with autolysosome formation (Fig. 2E). The rate of EGFR degradation was further monitored. Although PB did not alter the expression of EGFR at the 12 h treatment time point, it did downregulate EGFR at the 24 h and 48 h treatment time points, suggesting that PB-mediated lysosome nuclear localisation did not affect the degradation of endocytic cargo (Fig. 2F). Together, these results indicate that PB interferes with autophagosome fusion with lysosomes, or the formation of autolysosomes, but this interference has limited effects on the degradation of endocytic cargo.

PB-induced autophagy and -obstructed UPS results in Hep3B cell apoptosis

UPS is another cellular protein degradation system that is independent of lysosomal degradation. UPS and autophagy are interdependent38,39. Inhibition of autophagy will result in ubiquitinated protein aggregation39. Indeed, ubiquitinated protein levels were increased by PB, indicating that PB inhibited proteasome activity (Fig. 3A). In addition, the proteasome formation inhibitor MG132 did not significantly aggravate PB-augmented accumulation of ubiquitinated proteins (Fig. 3A), suggesting that PB itself saturates the ability of MG132 to inhibit protein degradation. These results indicated that PB also obstructed protein degradation through lysosome-independent UPS.

Figure 3. Simultaneous inhibition of autophagy and proteasome by PB triggers cell apoptosis.

Figure 3

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(A) Hep3B cells pre-treated with MG132 were treated for 6 h with the indicated concentrations of PB. Levels of protein expression were analysed by Western blot using antibodies against Ub and Actin. Images were cropped from different blots run under the same experimental conditions. The original blots were attached as Supplementary Figure 4A. (B) Hep3B and HepG2 cells treated for 24 h with indicated concentrations of PB. Fragment DNA in attached cells and medium was detected using ELISA kit. Data are presented as mean ± SD for 3 independent experiments. ***P < 0.001, control versus PB-treated cells. (C) Hep3B and HepG2 cells treated for 24 h with indicated concentrations of PB. Caspase activity of caspase 9, caspase 8, and caspase 3 were measured using specific chromo-substrates. Data are presented as mean ± SD for 3 independent experiments. ***P < 0.001, control versus PB-treated cells. (D) Hep3B and HepG2 cells were treated for 24 h with the indicated concentrations of PB. Levels of protein expression were analysed by Western blot using antibodies against intact or cleaved forms of caspase 3 and PARP as well as Actin. Images were cropped from different blots run under the same experimental conditions. The original blots were attached as Supplementary Figure 4B. (E) Hep3B and HepG2 cells pretreated with zVAD-fmk were treated for 24 h with indicated concentrations of PB. Fragment DNA in attached cells and medium was detected using ELISA kit. Data are presented as mean ± SD for 3 independent experiments. ***P < 0.001, control versus PB-treated cells. (F) Hep3B and HepG2 cells pretreated with MG132 were treated for 24 h with indicated concentrations of PB. Fragment DNA in attached cells and medium was detected using ELISA kit. Data are presented as mean ± SD for 3 independent experiments. **P < 0.01, ***P < 0.001, control versus PB-treated cells.

We next determined whether PB-induced autophagy and -obstructed UPS were associated with apoptosis. DNA fragmentation assays indicated that PB increased cellular apoptosis of attached cells in a dose-dependent manner, but only exerted limited effects in detached cells, suggesting that little anoikis occurred (Fig. 3B). In addition, PB effectively induced activities of caspase 8, caspase 9, and caspase 3 (Fig. 3C), indicating both extrinsic and intrinsic apoptotic pathways were activated. Western blotting further demonstrated increased expression levels of cleaved poly (ADP-ribose) polymerase 1(PARP1) and caspase 3 (Fig. 3D). Pretreatment of cells with a pan-caspase inhibitor, zVAD-fmk, almost completely blocked PB-induced DNA fragmentation (Fig. 3E). In contrast, pretreatment of cells with MG132 enhanced 60 and 120 μg/mL, but not 240 μg/mL, PB-induced DNA fragmentation (Fig. 3F). These results indicate that PB-induced autophagy and -obstructed UPS may subsequently trigger caspase-dependent apoptosis in Hep3B cells.

PB-triggered apoptosis occurred in an ER stress-related manner in Hep3B cells

General agreement suggests that one purpose for the upregulation of autophagy is to remove misfolded protein aggregates to ameliorate ER stress40,41,42. Once this fails, cells undergo apoptosis to minimize systemic damage. Death-associated protein kinase 3 (DAPK3) is an upstream integrator of apoptosis and autophagy in response to ER stress11. Indeed, DAPK3 was dramatically upregulated upon PB treatment, implying the involvement of ER stress in PB cytotoxic effects (Fig. 4A). To investigate whether ER stress was involved in PB-induced proteasome inhibition, cells treated with or without PB were stained with ubiquitin (Ub) and a dual functional protein disulphide isomerase (PDI), which has chaperone activity in the ER but redox properties outside the ER, and compared to those treated with MG132. As expected, confocal microscopy demonstrated different Ub distribution patterns in cells: a light vacuole pattern in untreated cells compared to a dense reticular pattern in PB treated cells. In addition, PDI showed a reticular pattern in untreated cells but a nuclear localisation pattern in PB treated cells. No similar observations were obtained in the MG132 group (Fig. 4B). ROS are frequently induced by herb-induced ER stress to synergistically trigger protein degradation inhibitor-mediated autophagy and apoptosis43. Induction of autophagy and obstruction of UPS may also induce intracellular ROS production. Therefore, the status of PB-induced ROS was measured. As expected, PB treatment induced high levels of ROS in Hep3B and HepG2 cells. Pretreatment of cells with a free radical scavenger NAC significantly reduced PB-induced ROS production (Fig. 4C) and PB-induced ubiquitinated protein accumulation (Fig. 4D), indicating NAC reduced the ER stress induced by PB. In addition, pretreatment with NAC downregulated endogenous EGFR and PB-induced ubiquitinated EGFR (Fig. 4D). These results suggested that PB deregulates proteasome formation and induces cell death in part via ROS-induced ER stress.

Figure 4. PB triggered cell apoptosis involves elevation of reactive oxygen species (ROS) and accumulation of ER stress.

Figure 4

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(A) Hep3B cells were treated for 6 h with the indicated concentrations of PB. Levels of protein expression were analysed by Western blot using antibodies against DAPK3 and Actin. Images were cropped from different blots run under the same experimental conditions. The original blots were attached as Supplementary Figure 5A. (B) Localisation of the ubiquitinated protein aggregates and ER stress and ROS-related protein PDI was imaged using a confocal microscope (Leica SP8). (C) Hep3B or HepG2 cells pretreated with or without 1 h N-acetyl-L-cysteine (NAC, 5 mM) were treated for 5 h with PB, or polyphenols gallic acid (GA), 3,4-dihydroxybenzoic acid (DHBA), and chlorogenic acid (CA), alone or in combination. Total reactive oxygen species (ROS) generation was quantified using DCFDA. Tert-butyl hydrogen peroxide (TBHP, 20 μM) was used as a positive control. Data are presented from three independent experiments. *P < 0.05, ***P < 0.001, NAC treated versus NAC untreated reactions. (D) Hep3B cells pre-treated with NAC were treated for 6 h with the indicated concentrations of PB. Levels of protein expression were analysed by Western blot using antibodies against Ub and Actin. Images were cropped from different blots run under the same experimental conditions. The original blots were attached as Supplementary Figure 5B.

PB inhibits cell cycle progression, cell-cell adhesion, and cell migration through the disruption of actin cytoskeleton organization

To examine the potential cancer killing potential of PB, two main mechanisms of cancer progression, cell cycle progression and cytoskeleton organization, were examined. Western blotting revealed that PB decreased cyclin and cyclin dependent kinase (CDK) levels in Hep3B and HepG2 cells (Fig. 5A). Flow cytometry revealed that PB treatment significantly increased apoptotic sub-G1 phase content, indicating an increase of DNA fragments after PB treatment (Figs 3C and 5B). These results indicate that PB deregulates the expression of cyclins and CDKs to perturb cell cycle progression, leading to hepatoma cancer cell apoptosis.

Figure 5. PB downregulates several proteins related to cell cycle progression, morphology, cell-cell adhesion and cell migration.

Figure 5

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Hep3B cells were treated for 24 h (A), 6 h (C), and 24 h and 48 h (F) with the indicated concentrations of PB. Levels of protein expression were analysed by Western blot using indicated antibodies. Images were cropped from different blots run under the same experimental conditions in each panel. The original blots were attached as Supplementary Figure 6. (B) Hep3B cells were treated for 8 or 24 h with 120 or 240 μg/mL PB. Cell cycle distribution was evaluated using propidium iodide (PI) staining. (D,E) Hep3B cells were treated for 6 h with PB (120 μg/mL), paclitaxel (PTX) (2.0 μM). Localisation of β-tubulin, F-actin and ezrin was imaged using a confocal microscope (Leica SP8). Differential expression of indicated molecules was compared. *P < 0.05; **P < 0.01, ***P < 0.001, control versus PB- or drug-treated cells. (G) Hep3B cells were treated for 1 h with indicated concentration of PB and adhered to the bottom of the plates. Adhesive cells were photographed and counted. (H) Hep3B cells were treated for 24 h with PB (60–240 μg/mL), the corresponding concentration of polyphenols GA, DHBA, and CA, along or in combination, or water control. Migration analysis was performed using a Boyden chamber. Migrated cells were measured as a percentage of cells that migrated to the lower surface of the chamber. Data are presented from three independent experiments. **P < 0.01; ***P < 0.001, control versus PB- or drug-treated cells.

Actin and microtubules provide a dynamic cellular framework to maintain and orchestrate cellular processes including motility. Indeed, downregulation of filament actin (F-actin) and its cell membrane-associated scaffold protein, Ezrin, was observed concomitantly after PB treatment (Fig. 5C). In addition, confocal microscopy analysis showed that PB treatment induced the alteration of F-actin and β-tubulin cytoskeleton organization in Hep3B cells (Fig. 5D and E). Furthermore, integrin αVβ1, which mediates actin cytoskeletal remodelling in response to force transmission orientation and dynamic stiffening or cyclic stretching of the extracellular matrix44, as well as N-cadherin and the transcription factors twist, slug, and snail, were all down-regulated in response to PB (Fig. 5F).

Nevertheless, functional analyses revealed that PB significantly restricted cell adhesion and migration in a dose-dependent manner (Fig. 5G and H). Wound closure following PB or control treatment were recorded using time-lapse microscopy, showing that PB significantly inhibited wound closure from 6 h after treatment (Supplementary Fig. S1A). The velocity and migrated distance of PB-treated cells were significantly reduced when compared with control-treated cells and even microtubule-stabilizing agent PTX-treated cells. Interestingly, the polyphenols GA, DHBA, and CA, alone or in combination, were involved in PB-restricted Hep3B cell migration (Supplementary Fig. S1B). These results show that PB restricts cancer cell adhesion and motility through PB-disrupted proteostasis. In addition, the polyphenols GA, DHBA, and CA also contribute to the inhibitory effects of PB on motility.

PB inhibits Hep3B and HepG2 cell growth in NOD-SCID xenograft mice

To translate the anti-HCC effects of PB in vivo, Hep3B and HepG2 xenograft mouse models were utilised. At 7 days post-cancer cell injection, mice were randomly assigned to vehicle or PB groups. All treatments were orally administered five times per week for 25 days. As demonstrated in Fig. 6A, PB administration significantly reduced tumour volume when compared with vehicle group in both Hep3B xenograft mice at day 35 (P = 0.045) or HepG2 xenograft mice at day 42 (P = 0.043). No significant weight loss was observed (Fig. 6B). These results indicate that PB potently attenuates tumour growth in Hep3B- and HepG2-bearing mice cells in vivo, without exerting severe toxic effects.

Figure 6. PB inhibits Hep3B and HepG2 xenograft growth in NOD-SCID mice without apparent toxicity.

Figure 6

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(A) Hep3B and HepG2 bearing mice were orally administrated either water or PB (5.8 mg lyophilised PB/100 μL of water/day) for 4 or 5 weeks. Tumour growth following PB treatment was significantly delayed in both Hep3B and HepG2 xenograft mice. (B) No difference in body weight was observed between Hep3B or HepG2 xenograft mice treated with (open circle) and without (closed circle) PB. Data are presented from at least four mice per group. *P < 0.05.

Transcriptome regulation by PB in Hep3B and HepG2 cells

To further examine the effect of PB in Hep3B and HepG2 cells, PB-induced changes in gene expression were determined by microarray analysis using a whole genome Human OneArray®. Genes significantly up- or down-regulated (P < 0.05) by PB are listed in Supplementary Table S1. The 50 genes that were most highly upregulated and most heavily downregulated by PB treatment were chosen to construct a heat map (Supplementary Fig. 2A). The enriched top 5 association networks are listed in Table 1, and the top 1 network is shown in Supplementary Fig. 2B. CDK1 was shown as a key regulator in network 1. In addition, molecular analysis revealed that 11 upregulated and 7 downregulated molecules could be applied as biomarkers for diagnosis, therapeutic efficacy, disease progression, safety, prognosis, and response to therapy. Drug analysis revealed that PB has multiple targets, including TUBB3, AGER, DCK, and TOP2A, implying the potential of PB as an alternative medicine. Nevertheless, drug analysis further indicated the potential for co-treatment with PB and a SERPINE1 inhibitor such as drotrecogin alfa to enhance anti-cancer effects (Supplementary Table S2). The networks generated by Ingenuity pathway analysis software further supported that PB suppressed cancer through the inhibition of cellular growth and proliferation, inhibition of cellular development, promotion of cell death, disruption of the cell cycle, and blockage of cellular movement. In addition, it provided potential clinical applications that may merit further translational study.

Table 1. Top 5 functional networks established from common top 50 PB-upregulated and top 50 PB-downregulated genes in Hep3B and HepG2 cells.

ID Molecules in Network Score Focus Molecules Associated Network Functions
1 alcohol group acceptor phosphotransferase, Ap1, ATF3, C/ebp, CCNE2, CDC7, Cdk, CDK1, CDKN2B, CENPF, Ck2, Cyclin A, Cyclin D, CYP2B6, DCK, E2f, EGR1, ETS, GOLM1, JUNB, MIR17HG, NCF2, NEK2, NR1H4, PEPCK, RAD51AP1, Rb, RNA polymerase II, Rxr, SERPINE1, SKA2, SKP2, TACC2, TIMP1, TTK 47 22 Cell Cycle, Cellular Growth and Proliferation, Cellular Development
2 AGER, ARHGEF2, ASB9, Beta Tubulin, CDKN2C, DKK1, DNMBP, EML1, FES, GABRR1, hexokinase, Histone h3, Histone h4, HNRNPA2B1, Hsp27, Hsp70, Hsp90, IGFBP1, MAP1B, MX1, NES, NPC1, p85 (pik3r), PACRG, PDE6G, PSIP1, RAB8B, SMC4, TOP2A, TUBA1A, TUBA3C/TUBA3D, TUBB3, TUBB2B, tubulin (complex), tubulin (family) 31 16 Cancer, Connective Tissue Disorders, Organismal Injury and Abnormalities
3 ABCG5, ACO1, ADRB2, AKR1C1/AKR1C2, AKR1D1, BHLHE40, Bhlhe41, BTG1, CIPC, CLIC4, CPB2, CYP2B6, DGAT2, ENDOG, FDPS, GLYCTK, HIF1A, HNF4A, IGFBP1, LPIN1, LPIN2, NAALAD2, NR3C1, NUDT6, RASD1, REL, RORC, SLC39A14, Slco1a1, STK19, TXNL4B, UGT2A3, ZDHHC9, ZHX1, ZHX3 28 15 Carbohydrate Metabolism, Lipid Metabolism, Small Molecule Biochemistry
4 ABCB4, ACSS1, ACSS2, ADAM8, ASPM, CCND1, CDC14B, CDK4/6, CLIC4, CTDSP1, DUSP5, ERK1/2, ESR1, FBXO4, FST, GPAM, IL17RB, KLF9, mir-30, mir-183, MIR17HG, MYC, Ncoa6, NDRG2, NECTIN3, PHLDB3, PPARA, PTGER3, PTPRH, SP2, SPAG4, STXBP1, TP53, TP53INP2, ZFP36L1 26 14 Cell Morphology, Tissue Morphology, Cancer
5 ACO1, ANP32E, ARGLU1, CENPE, CMTR1, COLGALT1, FAM169A, FDPS, FOS, GRHPR, HELLS, HSPA4L, HSPB8, LMNA, MAP1A, MBNL3, MIB1, MICAL2, NTRK1, NXF1, PATL1, PAXBP1, PCF11, PCNP, POGZ, RAPGEF1, RBM6, SCAF8, SEMA3A, SEMA3G, SMG1, SSUH2, SYNE1, TAOK1, TMEM57 24 13 Cancer, Organismal Injury and Abnormalities, Cellular Development
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Discussion

In the present study, we report that PB elicited ER stress, which inhibited autophagosome and proteasome activity, resulting in apoptosis induction in Hep3B cells. In addition, cell adhesion and motility were restricted through the inhibition of integrins and cadherins, as well as the modulation of actin cytoskeleton organization. Furthermore, PB inhibited tumour growth without affecting body weight in xenografts. These results suggest inhibition of the autophagy and ubiquitin-proteosome systems rendered cells vulnerable to PB-initiated ER stress-triggered apoptosis. Our results agree with those of the previous reports for the chemicals present in this plant and further elucidate the cancer inhibitory effects of this plant extract29,30.

The relationship between autophagy and apoptosis is intricate. Accumulation of autophagy could induce the bulk degradation process, leading to apoptosis, whereas autophagy will be negatively regulated by the initiation of apoptosis45,46,47,48. We found that PB treatment for 6 h initiated the formation of autophagosomes and increased the expression of LC3B-II without inducing apoptosis. However, PB activated apoptotic pathways after 24 h treatment. Our results showed that PB-inhibited autophagy enhanced apoptosis and the anti-tumour effects of PB in vitro and in vivo.

The alternation of growth factors, cytoskeletons, and adhesion molecules during proteasome formation is considered as one strategy to change cell mobility and adhesion49,50. Indeed, more than 50% of clinical Western medicines originate from natural products, many of which have the ability to modulate cancer cells51. In addition, ROS has been shown to medicate chemopreventive agent-induced cancer cell death52. In this study, we found marked downregulation of EGFR, F-actin, and ezrin and reorganization of F-actin network, as well as downregulation of adhesion molecules and their upstream transcription factors in response to PB treatment. Moreover, the role of ROS in the induction of ER stress, followed by a dysregulation of proteostasis and subsequent apoptosis is expected53. Elevation of ROS leads to protein damage and degradation, but cancer cells may counteract stress through increasing their antioxidant defences to eliminate ROS54 or restore redox balance55, implying that PB may lower cancer cell antioxidant defences and thereby increasing the antitumor growth rate. These findings demonstrate that the anti-proliferative and anti-migratory activities of PB are at least in part due to ROS elevation. Future work should concern the development of an ideal PB-based anti-cancer formula based on the rules of the monarch/minister/assistant/guide in traditional Chinese medicine theory.

The therapeutic dose information generated in mice in this study could serve as a reference for future clinical studies by conversion to equivalent human intake information using the body surface area normalization method56. According to the conversion method, 276 mg/kg/day of PB in mice (~21 g body weight on average) would be ~1.4 g/day in human adult with 60 kg [276 mg/kg × 60 kg × Km factor for mice (3)/Km factor for humans (37)]57. This could be supplied by the consumption of herb extracts via dietary supplements.

In summary, we have demonstrated that PB induces cell death, inhibits cell adhesion, and restricts cell motility through a mechanism likely to involve activation of proteasome degradation and inhibition of protein synthesis in Hep3B cells. The present study provides new insights into the application of PB for HCC treatment.

Methods

Preparation and liquid chromatography–mass spectrometry/mass spectrometry (LC-MS/MS) analysis of PB

Aqueous extract of P. bistorta was obtained from Sun Ten Pharmaceutical Company (Taipei, Taiwan). First, 100 g of P. bistorta was boiled with 1.5 L of H2O at 100 °C for 30 min, then concentrated to 100 mL under reduced pressure, designed as P. bistorta soup. A clear supernatant was obtained by centrifugation at 12,000 rpm for 20 min and designed as PB. PB was estimated to contain a total of 58 mg of residues per mL by concentration in vacuo.

Gallic acid (GA, G7384), 3,4-dihydroxybenzoic acid (DHBA, 37580), chlorogenic acid (CA, C3878), and all other chemicals used were of analytical grade and obtained from Sigma-Aldrich. Retention time and MS/MS ion spectra of the standards were used to confirm polyphenol presence in PB extracts. The quantitation of the polyphenols in PB was performed using a standard addition method with LC-MS/MS analysis by monitoring their precursor ions. Briefly, a high-performance liquid chromatographic system (Ultimate 3000 LC; Dionex, Germany) coupled with a hybrid Q-TOF mass spectrometer (maXis impact; Bruker, Taiwan Co. Ltd) was applied, with chromatographic separation using an Atlantis T3 analytical column (C18, 5 μm, 2.1 × 150 mm; Waters, Millford, MA, USA). Mobile phase A consisted of 5% acetonitrile/0.1% formic acid, and mobile phase B consisted of acetonitrile/0.1% formic acid. A linear LC gradient was used from 5% (v/v) B to 99% B at a flow rate of 0.25 mL/min for 15 min. Between injection, a program consisting of a 9 min 99% phase B followed by a 40 min 1% phase B (v/v) was used to re-equilibrate the column. The ESI source was operated in the negative ion mode. Nitrogen was used for nebulizing (50 psi) and drying (8 L/min, 350 °C), and helium was used for collision. MS and MS/MS data were processed and extracted using DataAnalysis software 4.1 (Bruker).

Cell culture

All culture media were purchased from Thermo Fisher Scientific. The human hepatoma cancer cell lines Hep3B and HepG2, or primary mouse hepatocytes isolated from NOS-SCID mice were maintained respectively in Roswell Park Memorial Institute (RPMI) 1640 (31800-022), RPMI 1640, or Dulbecco’s modified Eagle medium (DMEM) (12100046) with 10% foetal bovine serum (16000044), 50 U/mL penicillin and 50 μg/mL streptomycin (15070063), and 2 mM L-glutamine (25030081) at 37 °C in a humidified atmosphere of 5% CO2.

Cell viability and imaging

5 × 103 Hep3B or HepG2 cells were seeded into each well of 96-well plates for 24 h. Then, the culture medium was replaced by the medium containing PB (0–2000 μg/mL), and the volume of each well is 100 μL. Following a further 24 h of culture, viable cells were estimated by measuring the conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (M2128, Sigma-Aldrich) to formazan crystals. Cell images were photographed using a live cell movie analyser (NanoEntek, Seoul, Korea).

Western blot analysis

Cells were lysed with lysis buffer (10 mM Tris, pH 7.5; 150 mM NaCl; 5 mM EDTA, pH 8.0; 0.1% sodium dodecyl sulphate (SDS); 1% deoxycholate; and 1% NP-40) supplemented with protease inhibitor cocktail (Roche). Cell lysates were subjected to SDS-polyacrylamide gel electrophoresis and then transferred to polyvinylidene fluoride membranes. After blocking with 5% skim milk, the membranes were incubated with primary antibodies and subsequently with appropriate peroxidase-conjugated secondary antibodies. Primary antibodies specific to p-mTOR, mTOR, p-4EBP1, p-p70S6K1 (#9862), 4EBP1 (#9452), p70S6K1 (#9202), caspase 3, cleaved caspase 3, poly (ADP-ribose) polymerase (PARP), cleaved PARP (#9915), cyclin B1 (CCNB1), CCND1, CCNE1 (#9869), ezrin (#3145), N-cadherin (#9961), integrin αV and integrin β1(#4749) were purchased from Cell Signaling Technology. Antibodies specific to EGFR (sc-03) and Ub (sc-8017) were purchased from Santa Cruz. Antibodies specific to Atg12 (GTX124181), p62/SQSTM1 (GTX100685), DAPK3 (GTX102404), CCNA2 (GTX103042), CDK1 (GTX110545), CDK2 (GTX101226), CDK4 (GTX102993), p27 (GTX100446), p21 (GTX100444), Snail1 (GTX100754), Slug (GTX121924), and Twist (GTX127310) were obtained from GeneTex, CA, USA. Antibodies specific to LC3 were purchased from Novus Biologicals, CO, USA (NB600-1384). Antibodies specific to actin were obtained from Millipore, CA, USA (MAB1501). Peroxidase-conjugated secondary antibodies were obtained from Jackson ImmunoResearch, PA, USA. Blots were developed using an enhanced chemiluminescence system (Thermo Fisher Scientific).

Immunofluorescence cell staining

For detecting LC3 pattern, Hep3B cells were transfected with pEGFP-LC3 (#24920, Addgene) for 24 h followed by treatment with PB (240 μg/mL) or water control for a further 24 h. For detecting EGFR distribution, cells were seeded onto four-chamber slides, treated accordingly, and fixed with 4% paraformaldehyde in PBS (phosphate-buffered saline, 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4), permeabilised with 0.1% Triton X-100 (X100, Sigma-Aldrich) in PBS, rinsed with PBS, and blocked with 5% bovine serum albumin (A2153, Sigma-Aldrich) in PBS and stained with primary antibodies for EGFR (sc-03, Santa Cruz), ezrin (#3145, Cell Signaling Technology), β-tubulin (sc-9104, Santa Cruz), or Ub (sc-8017, Santa Cruz), followed by Alexa-488 or Alexa-594-conjugated secondary antibody (ThermoFisher Scientific). Filament actin (F-actin) was stained with Alexa-594 conjugated phalloidin (A12381, ThermoFisher Scientific), and nuclei were stained using 4′,6-diamidino-2-phenylindole (DAPI) (D9542, Sigma-Aldrich). N-acetyl-L-cysteine (NAC) (A7250, Sigma-Aldrich) and Paclitaxel (PTX; 2.0 μM) were used accordingly. Fluorescence imaging was taken using a confocal laser scanning microscope (Leica TCS SP8, Leica microsystems, Wetzlar, Germany). Fluorescence intensities indicating an abundance of β-tubulin, F-actin, and ezrin were quantified using ImageJ software (National Institutes of Health, USA).

Detection of cell apoptosis

Cell apoptosis was quantitatively and qualitatively analysed by the following approaches: (i) Measure DNA fragmentation and histone release from nucleus using the Cell Death Detection ELISA PLUS kit (11774425001, Roche Diagnostics Ltd., Taipei, Taiwan) according to the manufacturer’s instructions. (ii) Caspase activity measurement of caspase 9, caspase 8, and caspase 3 using a Colorimetric Assay Kit according to the manufacturer’s instructions (BioVision) with LEHD-pNA (K119), IETD-p-nitroaniline (pNA) (K113), and DEVD-pNA (K106) as substrates, respectively. Fold-increase in caspase activity was determined by colorimetric signal normalised to cell viability followed by comparison of the results of treated samples with that of controls.

Cell cycle analysis

5 × 106 Hep3B cells were treated with PB for 8, 24, or 48 h, and then pelleted, and fixed overnight in ice-cold 70% ethanol in PBS. Cells were then washed twice with PBS and resuspended in 0.5 mL staining buffer consisted of 50 μg/mL propidium iodide (PI) (P4170, Sigma-Aldrich) and 10 μg/mL RNase A (R6513, Sigma-Aldrich) in PBS for 1 h incubation at room temperature in the dark, and followed by analysing using a FACSCaliber™ platform (Becton Dickinson Immunocytometry Systems, CA, USA). Data analysis was performed using FlowJo software (FlowJo, LLC, OR, USA).

Cell adhesion analysis

5 × 105 Hep3B cells were treated with indicated concentration of PB and seeded on 6-well plate for 1 h to allow the cells to adhere to the bottom of the plates, and the wells were washed with PBS and the cells were fixed in 4% paraformaldehyde. Adhesive cells were photographed and counted.

Transwell cell migration assay

24 h growth factor-reduced Hep3B cells were suspended in RPMI-1640 with 1% serum, and 1 × 105 cells were seeded into transwell Boyden chambers (8 μm pore, Millipore). Medium with 10% serum with PB (60–240 μg/mL), or a corresponding concentration of GA, DHBA, CA, or water control, was added into the lower chamber, and cells were cultured for 24 hrs. After fixation with 10% formaldehyde, cells were stained with 0.5% crystal violet (C0775, Sigma-Aldrich). Migrated cells on the underside of the membrane were photographed using the live cell movie analyser and evaluated using the Cell Counter plugin for ImageJ software.

Measurement of reactive oxygen species (ROS)

Cellular ROS was measured in Hep3B or HepG2 cells treated with PB (120 and 240 μg/mL), corresponding polyphenols, or water control for 5 h, with or without 1-h pretreatment with 5 mM NAC, by using a 2′-7′-dichlorofluorescin diacetate (DCFDA)-Cellular Reactive Oxygen Species Detection Assay Kit (ab113851, Abcam) according to the manufacturer’s protocol. Cells were analysed on a fluorescent plate reader (Berthold Technologies, TN, USA), and mean ± standard deviation was plotted for three replicates from each condition. Tert-butyl hydrogen peroxide (TBHP), which mimics ROS activity to oxidize DCFDA to fluorescent DCF, was used as positive control.

Mouse xenograft model

Five-week-old, female, non-obese diabetic/severe combined immunodeficiency (NOD-SCID) mice were obtained from Bio-LASCO Taiwan Co. Ltd (Taipei, Taiwan). All animal experiments were carried out according to regulations approved by the Laboratory Animal Center, National Taiwan University 20120013. For tumour growth experiments, 5 × 106 Hep3B or HepG2 cells were inoculated subcutaneously into the flanks of mice. Seven days after inoculation, mice were randomized into two groups and orally administered PB (232 mg/kg, n = 5 for Hep3B, n = 4 for HepG2) or water (n = 6 for Hep3B, n = 4 for HepG2), five times per week for a further 28 days (Hep3B cells) or 35 days (HepG2 cells). Tumour size was measured by calipers every 3 days, and tumour volume was determined using the following formula: width2 × length/2.

Statistical analysis

Statistical significance of differences between treatments was determined by two tailed Student’s t test. P < 0.05 was considered statistically significant.

Additional Information

How to cite this article: Liu, Y.-H. et al. Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells. Sci. Rep. 7, 41437; doi: 10.1038/srep41437 (2017).

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Material

Supplementary Information
srep41437-s1.pdf (5.6MB, pdf)

Acknowledgments

The authors would like to thank Sun Ten Pharmaceutical Company, Taipei, Taiwan for providing the extract of Polygonum bistorta, Hsin-Ying Tsai (visiting student from Master of Engineering Science, University of New South Wales, Australia at China Medical University) and Ju-Chun Tai (senior specialist at Office of Research and Development, China Medical University) for their technical assistance. This work is supported by Ministry of Science and Technology, Taiwan (MOST 101-2324-B-002-014-, MOST 102-2320-B-039-032 -MY3).

Footnotes

The authors declare no competing financial interests.

Author Contributions Y.H.L. and J.Y.L. conceived the experiment(s), Y.H.L., Y.P.W., H.Y.L., S.W.T., C.J.C., C.J.L. and C.Y.K. conducted the experiment(s), Y.H.L., Y.P.W., H.Y.L., S.W.T., C.J.C. and J.Y.L. analysed the results. All authors reviewed the manuscript.

References

  1. Llovet J. M., Burroughs A. & Bruix J. Hepatocellular carcinoma. Lancet 362, 1907–1917, doi: 10.1016/S0140-6736(03)14964-1 (2003). [DOI] [PubMed] [Google Scholar]
  2. Tang Z. Y. Hepatocellular carcinoma surgery–review of the past and prospects for the 21st century. Journal of surgical oncology 91, 95–96, doi: 10.1002/jso.20291 (2005). [DOI] [PubMed] [Google Scholar]
  3. Jemal A. et al. Global cancer statistics. CA: a cancer journal for clinicians 61, 69–90, doi: 10.3322/caac.20107 (2011). [DOI] [PubMed] [Google Scholar]
  4. Lam W. et al. PHY906(KD018), an adjuvant based on a 1800-year-old Chinese medicine, enhanced the anti-tumor activity of Sorafenib by changing the tumor microenvironment. Scientific reports 5, 9384, doi: 10.1038/srep09384 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Siegel R. L., Miller K. D. & Jemal A. Cancer statistics, 2015. CA: a cancer journal for clinicians 65, 5–29, doi: 10.3322/caac.21254 (2015). [DOI] [PubMed] [Google Scholar]
  6. Hipp M. S., Park S. H. & Hartl F. U. Proteostasis impairment in protein-misfolding and -aggregation diseases. Trends in cell biology 24, 506–514, doi: 10.1016/j.tcb.2014.05.003 (2014). [DOI] [PubMed] [Google Scholar]
  7. Boyce M. & Yuan J. Cellular response to endoplasmic reticulum stress: a matter of life or death. Cell death and differentiation 13, 363–373, doi: 10.1038/sj.cdd.4401817 (2006). [DOI] [PubMed] [Google Scholar]
  8. Yoshida H., Haze K., Yanagi H., Yura T. & Mori K. Identification of the cis-acting endoplasmic reticulum stress response element responsible for transcriptional induction of mammalian glucose-regulated proteins. Involvement of basic leucine zipper transcription factors. The Journal of biological chemistry 273, 33741–33749 (1998). [DOI] [PubMed] [Google Scholar]
  9. Oyadomari S. & Mori M. Roles of CHOP/GADD153 in endoplasmic reticulum stress. Cell death and differentiation 11, 381–389, doi: 10.1038/sj.cdd.4401373 (2004). [DOI] [PubMed] [Google Scholar]
  10. Maiuri M. C., Zalckvar E., Kimchi A. & Kroemer G. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nature reviews. Molecular cell biology 8, 741–752, doi: 10.1038/nrm2239 (2007). [DOI] [PubMed] [Google Scholar]
  11. Gozuacik D. et al. DAP-kinase is a mediator of endoplasmic reticulum stress-induced caspase activation and autophagic cell death. Cell death and differentiation 15, 1875–1886, doi: 10.1038/cdd.2008.121 (2008). [DOI] [PubMed] [Google Scholar]
  12. Eisenberg D. M. et al. Unconventional medicine in the United States. Prevalence, costs, and patterns of use. The New England journal of medicine 328, 246–252, doi: 10.1056/NEJM199301283280406 (1993). [DOI] [PubMed] [Google Scholar]
  13. Horneber M. et al. How many cancer patients use complementary and alternative medicine: a systematic review and metaanalysis. Integr Cancer Ther 11, 187–203, doi: 10.1177/1534735411423920 (2012). [DOI] [PubMed] [Google Scholar]
  14. Ye M. et al. Liquid chromatography/mass spectrometry analysis of PHY906, a Chinese medicine formulation for cancer therapy. Rapid communications in mass spectrometry: RCM 21, 3593–3607, doi: 10.1002/rcm.2832 (2007). [DOI] [PubMed] [Google Scholar]
  15. Lam W. et al. The four-herb Chinese medicine PHY906 reduces chemotherapy-induced gastrointestinal toxicity. Science translational medicine 2, 45ra59, doi: 10.1126/scitranslmed.3001270 (2010). [DOI] [PubMed] [Google Scholar]
  16. Tilton R. et al. A comprehensive platform for quality control of botanical drugs (PhytomicsQC): a case study of Huangqin Tang (HQT) and PHY906. Chinese medicine 5, 30, doi: 10.1186/1749-8546-5-30 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zhang W. et al. Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS). Journal of chromatography. A 1217, 5785–5793, doi: 10.1016/j.chroma.2010.07.045 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wang E. et al. Interaction of a traditional Chinese Medicine (PHY906) and CPT-11 on the inflammatory process in the tumor microenvironment. BMC medical genomics 4, 38, doi: 10.1186/1755-8794-4-38 (2011). [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rockwell S. et al. Preclinical studies of the Chinese Herbal Medicine formulation PHY906 (KD018) as a potential adjunct to radiation therapy. International journal of radiation biology 89, 16–25, doi: 10.3109/09553002.2012.717733 (2013). [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Farrell M. P. & Kummar S. Phase I/IIA randomized study of PHY906, a novel herbal agent, as a modulator of chemotherapy in patients with advanced colorectal cancer. Clinical colorectal cancer 2, 253–256, doi: 10.3816/CCC.2003.n.007 (2003). [DOI] [PubMed] [Google Scholar]
  21. Kummar S. et al. A phase I study of the chinese herbal medicine PHY906 as a modulator of irinotecan-based chemotherapy in patients with advanced colorectal cancer. Clinical colorectal cancer 10, 85–96, doi: 10.1016/j.clcc.2011.03.003 (2011). [DOI] [PubMed] [Google Scholar]
  22. Saif M. W. et al. Phase I study of the botanical formulation PHY906 with capecitabine in advanced pancreatic and other gastrointestinal malignancies. Phytomedicine: international journal of phytotherapy and phytopharmacology 17, 161–169, doi: 10.1016/j.phymed.2009.12.016 (2010). [DOI] [PubMed] [Google Scholar]
  23. Saif M. W. et al. First-in-human phase II trial of the botanical formulation PHY906 with capecitabine as second-line therapy in patients with advanced pancreatic cancer. Cancer chemotherapy and pharmacology 73, 373–380, doi: 10.1007/s00280-013-2359-7 (2014). [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yen Y. et al. Phase I/II study of PHY906/capecitabine in advanced hepatocellular carcinoma. Anticancer research 29, 4083–4092 (2009). [PubMed] [Google Scholar]
  25. Orban-Gyapai O., Lajter I., Hohmann J., Jakab G. & Vasas A. Xanthine oxidase inhibitory activity of extracts prepared from Polygonaceae species. Phytotherapy research: PTR 29, 459–465, doi: 10.1002/ptr.5275 (2015). [DOI] [PubMed] [Google Scholar]
  26. Liu Y., Staerk D., Nielsen M. N., Nyberg N. & Jager A. K. High-resolution hyaluronidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of anti-necrosis constituents in Chinese plants used to treat snakebite. Phytochemistry 119, 62–69, doi: 10.1016/j.phytochem.2015.09.005 (2015). [DOI] [PubMed] [Google Scholar]
  27. Zaidi S. F., Kim J. H., Tomoe Y., Usmanghani K. & Kadowaki M. Effect of Pakistani medicinal plants on IgE/antigen- and ionophore-induced mucosal mast cells degranulation. Pakistan journal of pharmaceutical sciences 27, 1041–1048 (2014). [PubMed] [Google Scholar]
  28. Li L., Huang Z., Xiao H., Chen X. & Zeng J. Effect of PBNA on the NO content and NOS activity in ischemia/reperfusion injury in the rat retina. Advances in experimental medicine and biology 664, 501–507, doi: 10.1007/978-1-4419-1399-9_57 (2010). [DOI] [PubMed] [Google Scholar]
  29. Manoharan K. P., Yang D., Hsu A. & Huat B. T. Evaluation of Polygonum bistorta for anticancer potential using selected cancer cell lines. Medicinal chemistry 3, 121–126 (2007). [DOI] [PubMed] [Google Scholar]
  30. Intisar A. et al. Anticancer constituents and cytotoxic activity of methanol-water extract of Polygonum bistorta L. African journal of traditional, complementary, and alternative medicines: AJTCAM/African Networks on Ethnomedicines 10, 53–59 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zhang Z. et al. Cancer chemopreventive activity of a mixture of Chinese herbs (antitumor B) in mouse lung tumor models. Oncogene 23, 3841–3850, doi: 10.1038/sj.onc.1207496 (2004). [DOI] [PubMed] [Google Scholar]
  32. Wang Y. et al. Chemopreventive effect of a mixture of Chinese Herbs (antitumor B) on chemically induced oral carcinogenesis. Molecular carcinogenesis 52, 49–56, doi: 10.1002/mc.20877 (2013). [DOI] [PubMed] [Google Scholar]
  33. Li W. et al. Fei-Liu-Ping ointment inhibits lung cancer growth and invasion by suppressing tumor inflammatory microenvironment. BMC Complement Altern Med 14, 153, doi: 10.1186/1472-6882-14-153 (2014). [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Liu R. et al. Anti-tumor enhancement of Fei-Liu-Ping ointment in combination with celecoxib via cyclooxygenase-2-mediated lung metastatic inflammatory microenvironment in Lewis lung carcinoma xenograft mouse model. Journal of translational medicine 13, 366, doi: 10.1186/s12967-015-0728-1 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Ravikumar B. et al. Regulation of mammalian autophagy in physiology and pathophysiology. Physiological reviews 90, 1383–1435, doi: 10.1152/physrev.00030.2009 (2010). [DOI] [PubMed] [Google Scholar]
  36. Kang R., Zeh H. J., Lotze M. T. & Tang D. The Beclin 1 network regulates autophagy and apoptosis. Cell death and differentiation 18, 571–580, doi: 10.1038/cdd.2010.191 (2011). [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Zhao J. H., Zhai B., Gygi S. P. & Goldberg A. L. mTOR inhibition activates overall protein degradation by the ubiquitin proteasome system as well as by autophagy. P Natl Acad Sci USA 112, 15790–15797, doi: 10.1073/pnas.1521919112 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Ciechanover A. Proteolysis: from the lysosome to ubiquitin and the proteasome. Nature reviews. Molecular cell biology 6, 79–87, doi: 10.1038/nrm1552 (2005). [DOI] [PubMed] [Google Scholar]
  39. Korolchuk V. I., Menzies F. M. & Rubinsztein D. C. Mechanisms of cross-talk between the ubiquitin-proteasome and autophagy-lysosome systems. FEBS letters 584, 1393–1398, doi: 10.1016/j.febslet.2009.12.047 (2010). [DOI] [PubMed] [Google Scholar]
  40. Ding W. X. et al. Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. The Journal of biological chemistry 282, 4702–4710, doi: 10.1074/jbc.M609267200 (2007). [DOI] [PubMed] [Google Scholar]
  41. Qin L., Wang Z., Tao L. & Wang Y. ER stress negatively regulates AKT/TSC/mTOR pathway to enhance autophagy. Autophagy 6, 239–247 (2010). [DOI] [PubMed] [Google Scholar]
  42. Verfaillie T., Salazar M., Velasco G. & Agostinis P. Linking ER Stress to Autophagy: Potential Implications for Cancer Therapy. International journal of cell biology 2010, 930509, doi: 10.1155/2010/930509 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Fribley A. & Wang C. Y. Proteasome inhibitor induces apoptosis through induction of endoplasmic reticulum stress. Cancer biology & therapy 5, 745–748 (2006). [DOI] [PubMed] [Google Scholar]
  44. Balcioglu H. E., van Hoorn H., Donato D. M., Schmidt T. & Danen E. H. The integrin expression profile modulates orientation and dynamics of force transmission at cell-matrix adhesions. Journal of cell science 128, 1316–1326, doi: 10.1242/jcs.156950 (2015). [DOI] [PubMed] [Google Scholar]
  45. Vaquero E. C., Rickmanni M. & Molero X. Balancing the mitochondrial crosstalk between apoptosis and autophagy. Autophagy 3, 652–654 (2007). [DOI] [PubMed] [Google Scholar]
  46. Eisenberg-Lerner A., Bialik S., Simon H. U. & Kimchi A. Life and death partners: apoptosis, autophagy and the cross-talk between them. Cell death and differentiation 16, 966–975, doi: 10.1038/cdd.2009.33 (2009). [DOI] [PubMed] [Google Scholar]
  47. Gordy C. & He Y. W. The crosstalk between autophagy and apoptosis: where does this lead? Protein Cell 3, 17–27, doi: 10.1007/s13238-011-1127-x (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Gong K., Zhang Z. X., Chen Y. C., Shu H. B. & Li W. H. Extracellular signal-regulated kinase, receptor interacting protein, and reactive oxygen species regulate shikonin-induced autophagy in human hepatocellular carcinoma. Eur J Pharmacol 738, 142–152, doi: 10.1019/j.ejphar.2014.05.034 (2014). [DOI] [PubMed] [Google Scholar]
  49. Ogburn K. D. & Figueiredo-Pereira M. E. Cytoskeleton/endoplasmic reticulum collapse induced by prostaglandin J2 parallels centrosomal deposition of ubiquitinated protein aggregates. The Journal of biological chemistry 281, 23274–23284, doi: 10.1074/jbc.M600635200 (2006). [DOI] [PubMed] [Google Scholar]
  50. Lee J. Y. et al. Curcumin induces EGFR degradation in lung adenocarcinoma and modulates p38 activation in intestine: the versatile adjuvant for gefitinib therapy. PloS one 6, e23756, doi: 10.1371/journal.pone.0023756 (2011). [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Cao Z. X. et al. Pogostone Induces Autophagy and Apoptosis Involving PI3K/Akt/mTOR Axis in Human Colorectal Carcinoma HCT116 cells. Journal of ethnopharmacology, doi: 10.1016/j.jep.2016.07.028 (2016). [DOI] [PubMed] [Google Scholar]
  52. Rigas B. & Sun Y. Induction of oxidative stress as a mechanism of action of chemopreventive agents against cancer. Brit J Cancer 98, 1157–1160, doi: 10.1038/sj.bjc.6604225 (2008). [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Heath-Engel H. M., Chang N. C. & Shore G. C. The endoplasmic reticulum in apoptosis and autophagy: role of the BCL-2 protein family. Oncogene 27, 6419–6433, doi: 10.1038/onc.2008.309 (2008). [DOI] [PubMed] [Google Scholar]
  54. Gorrini C., Harris I. S. & Mak T. W. Modulation of oxidative stress as an anticancer strategy. Nature reviews. Drug discovery 12, 931–947, doi: 10.1038/nrd4002 (2013). [DOI] [PubMed] [Google Scholar]
  55. Chondrogianni N. et al. Protein damage, repair and proteolysis. Molecular aspects of medicine 35, 1–71, doi: 10.1016/j.mam.2012.09.001 (2014). [DOI] [PubMed] [Google Scholar]
  56. Shannon R.-S., Minakshi N. & Nihal A. Dose translation from animal to human studies revisited. The FASEB Journal 22, 659–661, doi: 10.1096/fj.07-9574LSF (2008). [DOI] [PubMed] [Google Scholar]
  57. Reagan-Shaw S., Nihal M. & Ahmad N. Dose translation from animal to human studies revisited. Faseb J 22, 659–661, doi: 10.1096/fj.07-9574LSF (2008). [DOI] [PubMed] [Google Scholar]

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Supplementary Materials

Supplementary Information
srep41437-s1.pdf (5.6MB, pdf)

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