FIG 5 .

Biofilm elaboration in the rob deletion mutant requires a downstream gene(s) but not upstream genes. Bacteria were grown in TSB in the presence (Glc+) or absence (Glc-) of 1% glucose. Biofilm elaboration was measured using the polystyrene microtiter plate assay described in Materials and Methods. The averages and standard errors from each sample are shown. Δrob, FK300 rob deletion mutant; ΔrobΔUpstream, FK300 with deletions of rob and its upstream genes SAOUHC_ 2894, SAOUHC_ 2895, and SAOUHSC_2896; ΔrobΔDownstream, FK300 with the deletion of rob and its downstream genes SAOUHSC_2898, SAOUHSC_2899, and SAOUHSC_2900. WT, wild type.