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. 1991 Nov 15;88(22):10109–10113. doi: 10.1073/pnas.88.22.10109

A regulated RNA binding protein also possesses aconitase activity.

S Kaptain 1, W E Downey 1, C Tang 1, C Philpott 1, D Haile 1, D G Orloff 1, J B Harford 1, T A Rouault 1, R D Klausner 1
PMCID: PMC52877  PMID: 1946430

Abstract

A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.

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Selected References

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