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. 2016 Jul 24;7(33):52818–52831. doi: 10.18632/oncotarget.10810

Figure 1. Minocycline enhances the generation of DCs from BM cells.

Figure 1

A. DCs were generated from C57BL/6 mouse BM cells using GM-CSF (40 ng/ml) and IL-4 (40 ng/mL) without (Ctrl-DCs) or with indicated concentrations of minocycline (Mino-DCs) for 7 days. The data are presented as the mean ± SD of six independent experiments; **P < 0.01. B. Representative histograms showing CD11c expression in DCs generated with 5 μM minocycline. Solid line, Ctrl-DCs; shaded area, Mino-DCs; dotted line, isotype-matched control. C. DCs were generated from C57BL/6 mouse BM cells using GM-CSF and IL-4 with indicated concentrations of tetracycline antibiotics for 7 days (Deme: demeclocycline; Doxy: doxycycline; Tetr: tetracycline; Mino: minocycline). The data are presented as the mean ± SD of six independent experiments; * P < 0.05, **P < 0.01 compared with control.