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. 2016 Jul 23;7(33):53443–53458. doi: 10.18632/oncotarget.10803

Figure 3. miR-218 downregulates MACC1 expression.

Figure 3

(A) Expression of miR-218 in the transfected conditions was quantified using qRT-PCR. RNUB6 was used as internal control. (B) MACC1 mRNA and protein expression after transfection of control-miR, miR-218 and anti-miR-218 was determined using qRT-pCR and Western blotting. RPII was used as internal control for MACC1 mRNA expression and β-actin for Western blotting. Either miR-218 or anti-miR-218 did not regulate the MACC1 mRNA expression significantly, but mainly regulated the MACC1 at protein level, in these three cell lines. (C) Densitometric analysis of MACC1 protein bands normalized to β-actin represented as a mean value of triplicates, in comparison to the respective control. (*P < 0.05; **P < 0.01).