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. 1991 Nov 15;88(22):10134–10137. doi: 10.1073/pnas.88.22.10134

A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals.

D R Burton 1, C F Barbas 3rd 1, M A Persson 1, S Koenig 1, R M Chanock 1, R A Lerner 1
PMCID: PMC52882  PMID: 1719545

Abstract

A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of greater than 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.

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Selected References

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