Figure 1.

Gcn2 is required for nuclear Gln3-Myc¹³ localization in response to growth with nitrogen sources whose use requires NCR-sensitive transcription. Gln3-Myc¹³ localization was measured in (A) wild-type (BY4742) and (B) gcn2Δ cells cultured in complex YPD or YNB-minimal medium with glutamine (Gln), ammonia (Am.), or proline (Pro) as sole nitrogen source. Rapamycin (+Rap) or methionine sulfoximine (+Msx) were added where indicated to glutamine- or ammonia-grown cells, respectively. Intracellular Gln3-Myc¹³ was visualized and scored as cytoplasmic (red bars), nuclear-cytoplasmic (yellow bars), or nuclear (green bars) as described in Materials and Methods. Nucl.-Cyto., nuclear-cytoplasmic.