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. 2017 Jan 31;11(1):014109. doi: 10.1063/1.4975192

FIG. 1.

FIG. 1.

Schematic drawing of the self-priming compartmentalization (SPC) digital PCR chip and procedure of single cell digital PCR. (a) Photograph of the prototype of the SPC chip. It contains four individual panels, and suction chambers are on the top of the outlets. (b) Schematic diagram of the layered device structure of the chip, which is composed of two layers of PDMS (PDMS layer and Microarray layer), a nano-waterproof layer and two glass coverslip layers. (c) Diagram of the detail of the chip design. Thousands of microwells are connected with the main channel, green line represents the main channels and the red boxes represent the reaction microwells, the size of the microwells is 150 μm × 150 μm × 250 μm, the size of the main channel is 50 μm × 25 μm. (d) The process of the single cell gene expression digital PCR.