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. Author manuscript; available in PMC: 2017 Feb 6.
Published in final edited form as: Biochemistry. 2016 Jun 24;55(26):3667–3673. doi: 10.1021/acs.biochem.6b00114

Figure 4.

Figure 4

The N-terminal region of Cpx is necessary for the enhancement of content-mixing. Histogram of the content-mixing percentage among docked vesicle pairs triggered by 10 µM Ca2+ in the presence of 100 nM Cpx wild-type, Cpx 27 and Cpx M5E/K6E. Content-mixing with 100 nM Cpx and 20 µM VpS are also shown as a control. Error bars are standard deviations (S.D.) obtained from five independent data acquisitions with independently prepared samples.