Fig. 10.

TLR-mediated activation and NF-κB nuclear translocation are not accompanied by significant P-TEFb production induction in hµglia/HIV cells. a Representative Western blot analysis blots of CycT1 and CDK9 expression in whole cell extracts (WCE) from hµglia/HIV (HC01) and (HC69) cells treated with TLR agonists. Cells were incubated with indicated TLR agonists (Pam3CSK4 at 1 µg/mL, HKLM at 10⁸ cells/mL, poly (I:C) at 10 µg/mL, and LPS and flagellin at 5 µg/mL) for 16 prior to WCE preparation and SDS-PAGE/Western blot analysis with anti-CycT1 antibody, anti-CDK9 antibody, or anti-Tubulin antibody as loading control. b Bar graph depicts the relative level of CycT1 (black bars) and CDK9 (red bars) expression in each clone, with error bars representing the standard deviation of three experiments