Table 2.
PCR primers.
| Gene | NCBI-RS | Protein | Primer Pair (5′-3′) | Tm °C |
|---|---|---|---|---|
| Rn45s | NR_046239.1 | Fw: GTTCCGCTCACACCTCAGAT Rv: CAAGTGCGTTCGAAGTGTCG |
58 | |
| Lcat | NM_017024.2 | LCAT | Fw: ACACAGGCCAAGACTTCGAG Rv: GGTTGGGGACTTAGGAGTGC |
56 |
| ApoA1 | NM_012738.1 | ApoA-1 | Fw: CCTGGACAACTGGGACACTC Rv: GCCCAGAACTCCTGAGTCAC |
57 |
| Lipc | NM_012597.2 | HL | Fw: GCACTATGCTATTGCCGTGC Rv: TTGATGCCCACACTCAGACC |
60 |
| Srb1 | NM_031541.1 | SR-B1 | Fw: CCCCATGAACTGTTCCGTGA Rv: GATCTTCCCTGTTTGCCCGA |
57 |
National Center for Biotechnology Information (NCBI) reference sequence (RS); genes: Rattus norvegicus 45S pre-ribosomal RNA (Rn45s), lecithin cholesterol acyltransferase (Lcat), apolipoprotein A1 (Apoa1), lipase C hepatic type (Lipc) and scavenger receptor class B, member 1 (Scarb1) mRNA. Primer forward (Fw) and reverse (Rv).