Figure 7. KB reduces inflammation caused by LPS and protects HCs.

HCs from WT A. or TLR4^−/− B. mice were treated with LPS (1 μg/ml) preincubated with or without KB (15 μM). Supernatants were collected at 0.5, 1, 2 or 4 h. TNF-α levels were detected by ELISA and ALT activity was detected via automatic biochemical analyzer. Data are the result of three replicates and are expressed as means ± SD. #P < 0.01 vs. LPS group.