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. 2016 Aug 5;7(36):57821–57831. doi: 10.18632/oncotarget.11067

Figure 2. Vector-mediated UHRF1 overexpression caused DNA hypomethylation in ESCC cell lines.

Figure 2

(A) Western blot analysis of UHRF1 expression in KYSE30 cells treated with GFP-fused UHRF1 vector. (B) Vector-mediated UHRF1 overexpression caused LINE-1 hypomethylation (P = 0.005). (C) Pyrograms for LINE-1 methylation levels in KYSE30. (D, E) Changes in DNA methylation after co-transfection with UHRF1 vector and EGFP-MBD1 vector. (F) Expression of EGFP-MBD1 after co-transfection determined by western blot analysis.