Extended Data Table 1. a Data collection and refinement statistics.
| Cez apo SeMet (88-438) | Cez apo (129-438) | Cez~Ub (129-438, QPG) | Cez~Lys11 diUb (129-438) | A20~Ub (1-366) | ||
|---|---|---|---|---|---|---|
| Data collection | ||||||
| Space Group | P41212 | P41212 | H3 | P212121 | P1211 | |
| Cell dimensions | ||||||
| a, b, c (Å) | 96.72, 96.72, 83.37 | 103.35, 103.35, 90.20 | 157.56, 157.56, 75.6 | 92.03, 56.52, 91.75 | 64.20, 71.95, 203.93 | |
| α, β, γ (Å) | 90, 90, 90 | 90, 90, 90 | 90, 90, 120 | 90, 90, 90 | 90, 94.64, 90 | |
| Resolution (Å) | 50.00 - 3.70 (3.83 - 3.70) | 90.20 - 2.20 (2.27 - 2.20) | 66.13 - 2.00 (2.05 - 2.00) | 56.52 - 2.80 (2.87 - 2.80) | 49.33 - 2.85 (2.96 - 2.85) | |
| Rmerge | 0.212 (0.718) | 0.071 (0.829) | 0.125 (0.854) | 0.145 (0.689) | 0.070 (0.384) | |
| < I/σI > | 11.7 (4.3) | 13.1 (1.9) | 8.6 (1.9) | 9.5 (2.0) | 9.6 (2.1) | |
| CC1/2 | 0.985 (0.437) | 0.994 (0.515) | 0.990 (0.691) | 0.995 (0.867) | ||
| Completeness (%) | 99.8 (100) | 100 (99.9) | 100 (100) | 94.6 (95.5) | 99.4 (99.8) | |
| Redundancy | 7.7 (7.6) | 7.9 (5.8) | 4.2 (4.1) | 4.6 (4.6) | 3.2 (3.3) | |
| Refinement | ||||||
| Resolution (Å) | 73.08 - 2.20 | 45.48 - 2.00 | 48.16 - 2.80 | 49.33 - 2.85 | ||
| No. reflections | 25351 | 47215 | 11478 | 43393 | ||
| Rwork / Rfree | 19.8 / 21.9 | 17.6 / 21.7 | 20.7 / 24.4 | 19.5/ 24.6 | ||
| No. atoms | ||||||
| Protein | 2143 | 5599 | 3318 | 11558 | ||
| Ligand/ion | - | 18 | 35 | 8 | ||
| Water | 90 | 621 | 3 | - | ||
| B factors | ||||||
| Protein | 72.3 | 29.0 | 46.8 | 67.1 | ||
| Ligand/ion | - | 36.9 | 56.3 | 67.6 | ||
| Water | 58.8 | 34.2 | 17.2 | - | ||
| R.m.s deviations | ||||||
| Bond length (Å) | 0.002 | 0.002 | 0.002 | 0.002 | ||
| Bond angles (°) | 0.59 | 0.62 | 0.52 | 0.54 | ||
Values in parentheses are for the highest resolution shell. All datasets were collected from a single crystal each.