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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Nanomedicine. 2016 Aug 9;13(2):443–454. doi: 10.1016/j.nano.2016.07.015

Figure 3.

Figure 3

Flow cytometry analysis of JAWSII dendritic cells treated with the AF647-KLH conjugate or AF647-labeled nanovaccine NPs of different sizes for 2 h. (A), (D) and (B), (E) show the intensity distribution and mean intensity of AF647 fluorescence, respectively, in dendritic cells treated with AF647-KLH or AF647-labeled nanovaccines. (C) Recorded events show that more than 99% of cells were labeled for both 100 and 500 nm nanovaccine particles. AF647 was conjugated to KLH to form the AF647-KLH conjugate. AF647-KLH was associated to the surface of hybrid NPs to form fluorescent nanovaccine NPs. The blank group are cells that were not treated with NPs. Quantitative data are expressed as means ± SD.