Table 1.

Physicochemical properties of NPs.

NPs	Size (d. nm)	Zeta potential (mV)	PDI	Nic-KLH conjugation efficiency (%)
PLGA NP-100 nm	98.5 ± 7.2	-4.32 ± 0.24	0.23 ± 0.03	--
PLGA NP-500 nm	451.6 ± 14.0	-4.94 ± 0.34	0.24 ± 0.03	--
Liposome	110.7 ± 4.5	10.70 ± 0.78	0.19 ± 0.02	--
Hybrid NP-100 nm	102.1 ± 4.5	9.60 ± 0.47	0.21 ± 0.03	--
Hybrid NP-500 nm	456.9 ± 7.8	10.10 ± 0.58	0.22 ± 0.04	--
Nanovaccine-100 nm	108.7 ± 3.7	2.29 ± 0.31	0.20 ± 0.02	88.28 ± 0.49
Nanovaccine-500 nm	467.5 ± 10.3	2.69 ± 0.07	0.23 ± 0.03	88.53 ± 2.02