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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Methods. 2016 Sep 21;113:105–110. doi: 10.1016/j.ymeth.2016.09.011

Figure 2.

Figure 2

Nuclear import of SerRS tested by cell fractionation assay. HEK293 cells (0.5–1×107) were treated with UV (6 mJ/cm2) irradiation or not treated before the whole cell lysates (WCL) were prepared and the nuclear (N) and cytoplasmic (C) fractions were extracted. The WCL, N and C fractions were further analyzed by Western blot. WCL was blotted to evaluate the effect of the treatment on the expression of the AARS. Blotting cytoplasmic specific protein α-tubulin and nuclear specific protein lamin A/C was used to evaluate the purities of the fractions. In this experiment, there were small amounts of cytoplasmic contamination in the nuclear fractions as indicated by the presence of α-tubulin. The contamination can be removed by additional wash of the nuclear pellets with PBS buffer after the cytoplasmic fractions is removed.