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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Nature. 2017 Feb 1;542(7640):242–245. doi: 10.1038/nature21080

Figure 4. Dietary AHR ligands restore immunity to C. rodentium.

Figure 4

a, Survival plot C. rodentium infected R26Cyp1a1 mice fed control or I3C diet (n=6 per group). b, C. rodentium burdens in faeces (Ctrl: n=5, I3C: n=6). c, Colon sections of C. rodentium infected mice stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue). Scale bars represent 100μm. d, Representative photomicrographs of H&E stained colon sections. Scale bars represent 100μm. e, Pathology scores. Bars show the mean and symbols represent individual mice. f, Absolute numbers of cytokine-producing TCRβ+CD4+ T cells in the colon of C. rodentium infected mice (n=4 per group), mean ± s.e.m.. g, IL-17A and IL-22 expression in TCRβ+CD4+ T cells from the colon of C. rodentium infected mice. h, Survival plot of R26Cyp1a1 mice fed I3C diet treated with anti-IL-22 blocking antibody (n=6) or isotype control (n=5). i, Concentration-dependent inhibition of human recombinant CYP1A1 enzyme activity by I3C, DIM and FICZ.

Results are representative of three independent experiments. Error bars, mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, as calculated by Student’s t-test.