Table 1.
Identification of proteins differentially expressed after low dose chronic exposure in normal human fibroblast VH10 cells.
| Spot Number | Protein Name | Protein ID | Theoretical pI | Theoretical Mr, kDa | Peptide Matches | Sequence Coverage % | Mascot Score | Protein Function |
|---|---|---|---|---|---|---|---|---|
| 1 | α-Enolase | P06733 | 7.0 | 47.2 | 15 | 36 | 120 | Glycolysis |
| 2 | α-Enolase | P06733 | 7.0 | 47.2 | 20 | 33 | 197 | Glycolysis |
| 3 | Proliferation-associated protein 2G4 | Q9UQ80 | 6.1 | 43.8 | 14 | 27 | 66 | Involved in cell cycle arrest/cell proliferation |
| 4 | 26S protease regulatory subunit 8 | P62195 | 7.1 | 45.6 | 12 | 36 | 85 | Proteasome complex |
| 5 | Mannose-6-phosphate receptor-binding protein 1 | O60664 | 5.8 | 28.1 | 15 | 48 | 141 | Vesicle-mediated transport |
| 6 | Serine-threonine kinase receptor-associated protein | Q9Y3F4 | 5.0 | 38.4 | 9 | 41 | 70 | mRNA processing, regulator of TGFβ pathway, cofactor of p53 |
| 7 | Serine-threonine kinase receptor-associated protein | Q9Y3F4 | 5.0 | 38.4 | 8 | 32 | 65 | mRNA processing, regulator of TGFβ pathway, cofactor of p53 |
| 8 | Alcohol dehydrogenase | P14550 | 6.3 | 36.6 | 13 | 55 | 110 | Glucose metabolic process |
| 9 | LIM and SH3 domain protein 1 | Q14847 | 6.4 | 30.1 | 10 | 31 | 75 | Actin-binding protein |
| 10 | Eukaryotic translation initiation factor 3 | Q13347 | 5.4 | 36.5 | 9 | 30 | 71 | Protein biosynthesis |
| 11 | Eukaryotic translation initiation factor 3 | Q13347 | 5.4 | 36.5 | 10 | 32 | 73 | Protein biosynthesis |
| 12 | F-actin-capping protein α-1 | P52907 | 5.4 | 32.9 | 14 | 67 | 172 | Regulation of cell motility |
| 13 | Tubulin beta | P07437 | 4.8 | 48.7 | 14 | 45 | 132 | Cytoskeleton |
| 14 | Annexin A1 | P04083 | 6.6 | 38.7 | 18 | 52 | 179 | Regulation of apoptosis |
| 15 | Annexin A1 | P04083 | 6.6 | 38.7 | 19 | 62 | 202 | Regulation of apoptosis |
| 16 | PDZ and LIM domain protein 1 | O00151 | 6.6 | 36.5 | 9 | 23 | 78 | Cytoskeleton protein required for actin stress fiber formation |
| 17 | F-actin-capping protein subunit β | P47756 | 5.4 | 31.5 | 9 | 44 | 67 | Actin-binding protein |
| 18 | Chloride intracellular channel protein CLIP1 | O00299 | 5.1 | 27.4 | 16 | 70 | 183 | Chloride ion channel, anti-apoptotic |
| 19 | Annexin A2 | P07355 | 7.6 | 38.6 | 20 | 51 | 210 | Stress response, regulation of apoptosis |
| 20 | Glutathione transferase omega-1 | P78417 | 6.2 | 27.8 | 10 | 29 | 100 | Metabolism of xenobiotics, antioxidant |
| 21 | Heat shock protein β-1 (Hsp27) | P04792 | 6.0 | 22.8 | 8 | 38 | 65 | Involved in stress resistance and actin organization |
| 22 | Phosphoglycerate mutase | P18669 | 6.4 | 26.7 | 18 | 73 | 200 | Glycolysis |
| 23 | Ubiquitin thiolesterase L1, acidic isoforms | P09936 | 5.3 | 24.8 | 14 | 72 | 131 | Processing of ubiquitinated proteins; anti-apoptotic |
| 24 | Ubiquitin thiolesterase L1, basic isoforms | P09936 | 5.3 | 24.8 | 14 | 75 | 171 | Processing of ubiquitinated proteins; anti-apoptotic |
| 25 | Peroxiredoxin 6 | P30041 | 6.0 | 25.0 | 7 | 36 | 57 | Antioxidant |
| 26 | Peroxiredoxin 6 | P30041 | 6.0 | 25.0 | 13 | 64 | 144 | Antioxidant |
| 27 | Peroxiredoxin 6 | P30041 | 6.0 | 25.0 | 14 | 70 | 155 | Antioxidant |
| 28 | Triosephosphate isomerase | P60174 | 6.4 | 26.7 | 15 | 58 | 177 | Carbohydrate metabolism |
| 29 | 26S proteasome subunit 10 | O75832 | 5.4 | 20.4 | 8 | 49 | 58 | Acts as a regulatory subunit of the 26S proteasome |
| 30 | Glutathione S-transferase P | P09211 | 5.4 | 23.6 | 9 | 48 | 94 | Antioxidant, anti-apoptotic |
| 31 | Glutathione S-transferase P | P09211 | 5.4 | 23.6 | 10 | 56 | 146 | Antioxidant, anti-apoptotic |
| 32 | Protein DJ-1 | Q99497 | 6.3 | 19.9 | 8 | 43 | 60 | Redox-sensitive chaperone and a sensor for oxidative stress |
| 33 | Proteasome subunit α type-2 | P25787 | 6.9 | 26.0 | 8 | 44 | 70 | Proteasome complex |
| 34 | Acyl-protein thioesterase 1 | O75608 | 6.3 | 26.7 | 5 | 41 | 56 | De-palmitoylation of signaling proteins |
| 35 | Superoxide dismutase Mn SOD2 | P04179 | 8.3 | 24.7 | 7 | 36 | 69 | Antioxidant, age-dependent response to ROS |
| 36 | Superoxide dismutase Mn SOD2 | P04179 | 8.3 | 24.7 | 8 | 40 | 78 | Antioxidant, age-dependent response to ROS |
| 37 | Peroxiredoxin-2 | P32119 | 5.7 | 21.9 | 9 | 35 | 79 | Antioxidant, anti-apoptotic |
| 38 | Adenine phosphoribosyltransferase | P07741 | 5.8 | 19.6 | 8 | 68 | 91 | Nucleotide metabolism |
| 39 | Nucleoside diphosphate kinase A | P15531 | 5.8 | 17.3 | 8 | 61 | 90 | Synthesis of nucleoside triphosphates other than ATP, tumor suppressor, cofactor of p53 |
| 40 | Transgelin | Q01995 | 8.9 | 22.6 | 13 | 56 | 121 | Actin-binding protein, senescence marker |
The spot numbers refer to Figure 2A. Protein ID number and predicted protein function are found at UniProtKB [25]. The numbers of identified peptides matching predicted peptides and coverage of the entire protein sequence by the identified tryptic peptides are presented. Mascot Scores greater than 56 are considered significant (p ≤ 0.05).