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. 1991 Dec 1;88(23):10961–10965. doi: 10.1073/pnas.88.23.10961

M-15: high-affinity chimeric peptide that blocks the neuronal actions of galanin in the hippocampus, locus coeruleus, and spinal cord.

T Bartfai 1, K Bedecs 1, T Land 1, U Langel 1, R Bertorelli 1, P Girotti 1, S Consolo 1, X J Xu 1, Z Wiesenfeld-Hallin 1, S Nilsson 1, et al.
PMCID: PMC53052  PMID: 1720557

Abstract

The 20-amino acid peptide M-15 binds with high affinity (IC50 approximately 0.1 nM) to 125I-labeled galanin (125I-GAL) binding sites in membranes from the ventral hippocampus, midbrain, and rat spinal cord. Receptor autoradiographic studies show that M-15 can displace 125I-GAL from all labeled sites. M-15 acts as a reversible high-affinity antagonist in blocking the inhibitory effects of GAL on the evoked release of acetylcholine in vivo in the hippocampus and on the GAL-induced hyperpolarization of locus coeruleus neurons in slices. M-15 also blocks the facilitatory effects of GAL on the spinal flexor reflex. Thus, the chimeric peptide M-15 [GAL-(1-13)-substance P-(5-11)amide] represents the first antagonist to the neuronal actions of GAL.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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