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. 1991 Dec 15;88(24):11091–11094. doi: 10.1073/pnas.88.24.11091

Uncoupling thermotolerance from the induction of heat shock proteins.

B J Smith 1, M P Yaffe 1
PMCID: PMC53079  PMID: 1763024

Abstract

Exposure of cells to elevated temperatures causes a rapid increase in the synthesis of heat shock proteins (hsps) and induces thermotolerance, the increased ability of cells to survive exposure to lethal temperatures; however, the connection between hsp induction and the acquisition of thermotolerance is unclear. hsp induction in the yeast Saccharomyces cerevisiae is mediated by the activation of heat-shock transcription factor, and recently we have described a mutation, hsf1-m3, in heat-shock transcription factor that prevents the factor's activation. We now demonstrate that this mutation results in a general block in heat-shock induction but does not affect the acquisition of thermotolerance. Our results indicate that high-level induction of the major hsps is not required for cells to acquire thermotolerance.

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Selected References

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