Table 5. The median survival time and 2-year overall survival rates of the patients with R0 resection in survival analyses in this study.

Markers	Groups	Patients in IHC	Immunohistochemistry		Patients in qPCR	Quantitative PCR
		Follow-up rate (%)	Median survival time (months)	2-year overall survival rate	Follow-up rate (%)	Median survival time (months)	2-year overall survival rate
	All	325/377, 86.2%	65.4	75.1%	80/93, 86.0%	—	68.8%
Lgr5	Low	233/276, 84.4%	68.2	77.2%	45/50, 90.0%	30.4	60.0%
	High	93/101, 92.1%	55.3	70.0%	35/43, 81.4%	—	80.0%
Oct4	Low	235/266, 88.3%	69.7	78.3%	38/44, 86.4%	32.6	65.8%
	High	90/111, 81.1%	54.1	66.7%	42/49, 85.7%	—	71.4%
CD133	Low	271/311, 87.1%	68.2	75.3%	59/69, 85.5%	—	78.0%
	High	54/66, 81.8%	46.6	74.1%	21/24,87.5%	18.0	42.9%
EpCAM	Low	109/123, 88.6%	—	80.7%	55/65,84.6%	—	74.5%
	High	216/254, 85.0%	54.5	72.2%	25/28, 89.3%	29.1	56.0%
CD54	Low	281/321, 87.5%	68.0	75.8%	45/53,84.9%	—	73.3%
	High	44/56, 78.6%	36.0	74.5%	35/40, 87.5%	32.3	62.9%
Sox2	Low	180/211, 85.3%	59.0	71.1%	38/43, 88.4%	32.8	63.2%
	High	84/96, 87.5%	—	79.8%	42/50, 84.0%	—	73.8%