Figure 2. Antiproliferative effects of Amblyomin-X on Renca cells.
Renca cells were seeded in six-well plates (2 × 105 cells/well), incubated with 5 μM CFDA-SE for 15 min and, then, treated with Amblyomin-X (0.5 μM) for 24 h. The cellular CFDA-SE fluorescence intensity was estimated by flow cytometry. (A) Histograms considering each daughter cells generation, different color peaks indicated generations of daughter cells. (B) Bar plots of proliferation index regarding parental cells was calculated by using a software (WinMDI Version 2.8), regarding three independent experiments. *p ≤ 0.05, **p ≤ 0.005 compared to the control, as determined by unpaired t-tests with Welch's correction.