Table 1.
Top 30 antigens that discriminate between seronegatives and T. gondii infections. Antigens are ranked by descending protein array signal intensity (see Fig. 2 for details). pBH, Benjamini–Hochberg corrected P value; AUC, area under ROC curve
| Rank | Antigen (gene ID_exon) | Description | pBH | AUC | Sensitivity (%) | Specificity (%) | |
|---|---|---|---|---|---|---|---|
|
| |||||||
| Original nomenclature | New nomenclature | ||||||
| 1 | 054720_1 | 254720_1 | Dense granule protein 8 (GRA8) | 7·94E-18 | 0·989 | 98·9 | 100·0 |
| 2 | 070240_3 | 270240_3 | Unspecified product (MAG1) | 1·85E-14 | 0·972 | 93·3 | 100·0 |
| 3 | 109590_1 | 309590_3 | Rhoptry protein 1 (ROP1) | 1·46E-17 | 0·990 | 98·9 | 100·0 |
| 4 | 075440_1 | 275440_1 | Dense granule protein 6 (GRA6) | 6·48E-18 | 0·989 | 97·8 | 100·0 |
| 5 | 003310_1 | 203310_1 | Dense granule protein 7 (GRA7) | 5·01E-21 | 0·993 | 98·9 | 100·0 |
| 6 | 039740_1 | 239740_1 | Dense granule protein 14 (GRA14) | 1·57E-16 | 0·973 | 91·1 | 100·0 |
| 7 | 004050_8 | 204050_8 | Subtilisin (SUB1) | 0·006635 | 0·864 | 94·4 | 77·8 |
| 8 | 027280_1 | 227280_1 | Dense granule protein 3 (GRA3) | 1·43E-16 | 0·973 | 93·3 | 100·0 |
| 9 | 022370_1 | 222370_1 | SAG-related sequence 13 (SRS13) | 1·62E-05 | 0·833 | 80·0 | 77·8 |
| 10 | 110780_1 | 310780_1 | Dense granule protein 4 (GRA4) | 3·52E-13 | 0·933 | 87·8 | 100·0 |
| 11 | 026380_1 | 226380_1 | Hypothetical protein | 0·005644 | 0·722 | 62·2 | 88·9 |
| 12 | 058660_2 | 258660_2 | Rhoptry protein 6 (ROP6) | 1·11E-06 | 0·915 | 83·3 | 100·0 |
| 13 | 091890_4 | 291890_4 | Microneme protein 1 (MIC1) | 1·46E-10 | 0·907 | 84·4 | 100·0 |
| 14 | 067680_8 | 267680_8 | Microneme protein 12 (MIC12) | 1·27E-11 | 0·977 | 93·3 | 100·0 |
| 15 | 001780_2 | 201780_2 | Microneme protein 2 (MIC2) | 8·94E-09 | 0·899 | 80·0 | 100·0 |
| 16 | 033450_1 | 233450_1 | SAG-related sequence 29A (SRS29A) | 7·75E-08 | 0·767 | 66·7 | 100·0 |
| 17 | 053330_2 | 253330_2 | Rhoptry kinase family protein, truncated (incomplete catalytic triad) | 1·96E-05 | 0·753 | 60·0 | 100·0 |
| 18 | 051540_2 | 251540_2 | Dense granule protein 9 (GRA9) | 0·00022 | 0·870 | 78·9 | 100·0 |
| 19 | 020950_1 | 220950_1 | Hypothetical protein | 8·89E-05 | 0·644 | 52·2 | 100·0 |
| 20 | 111220_1 | 3112201 | Hypothetical protein | 6·68E-06 | 0·754 | 63·3 | 100·0 |
| 21 | 088960_2.S1 | 288960_2.S1 | Hypothetical protein | 8·94E-05 | 0·678 | 46·7 | 100·0 |
| 22 | 026660_15 | 226660_15 | Hypothetical protein | 0·034748 | 0·731 | 60·0 | 100·0 |
| 23 | 014080_1 | 214080_1 | Toxofilin | 1·88E-06 | 0·843 | 77·8 | 88·9 |
| 24 | 058950_2 | 258950_2 | Lectin family protein | 8·66E-05 | 0·810 | 76·7 | 88·9 |
| 25 | 095700_6 | 295710_6 | HECT-domain (ubiquitin-transferase) domain-containing protein | 1·95E-05 | 0·540 | 45·6 | 100·0 |
| 26 | 094690_1 | 294690_1 | Rhomboid protease 5 (ROM5) | 0·041552 | 0·517 | 30·0 | 100·0 |
| 27 | 021710_4 | 221710_4 | TBC domain-containing protein | 0·004158 | 0·678 | 61·1 | 88·9 |
| 28 | 090680_8 | 290678_8 | Hypothetical protein | 0·000124 | 0·777 | 54·4 | 100·0 |
| 29 | 033530_1 | 2335301 | Hypothetical protein | 3·51E-06 | 0·751 | 64·4 | 100·0 |
| 30 | 027620_2 | 227620_2 | Dense granule protein 2 (GRA2) | 1·14E-08 | 0·899 | 75·6 | 100·0 |